Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26818
Title: Recently differentiated epimastigotes from Trypanosoma cruzi are infective to the mammalian host.
Authors: Kessler, Rafael Luis
Contreras, Vıctor Tulio
Marliere, Newmar Pinto
Guarneri, Alessandra Aparecida
Silva, Luz Helena Villamizar
Mazzarotto, Giovanny Augusto Camacho Antevere
Batista, Michel
Soccol, Vanete Thomaz
Krieger, Marco Aurelio
Probst, Christian Macagnan
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Universidad de Carabobo. Facultad Ciencias de La Salud. Centro de Biologıa Molecular de Parasitos. Laboratorio de Protozoologıa.Valencia, Venezuela.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Universidad de Carabobo. Facultad Ciencias de La Salud. Centro de Biologıa Molecular de Parasitos. Laboratorio de Protozoologıa.Valencia, Venezuela.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.
Abstract: Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated by the widely acknowledged fact that only this stage is susceptible to the complement system. In the present work, we establish a T. cruzi in vitro and in vivo epimastigogenesis model to analyze the biological aspects of recently differentiated epimastigotes (rdEpi). We show that both trypomastigote stages of T. cruzi (cell-derived and metacyclic) are able to transform into epimastigotes (processes termed primary and secondary epimastigogenesis, respectively) and that rdEpi have striking properties in comparison to long-term cultured epimastigotes: resistance to complement-mediated lysis and both in vitro (cell culture) and in vivo (mouse) infectivity. Proteomics analysis of all T. cruzi stages reveled a cluster of proteins that were up-regulated only in rdEpi (including ABC transporters and ERO1), suggesting a role for them in rdEpi virulence. The present work introduces a new experimental model for the study of host-parasite interactions, showing that rdEpi can be infective to the mammalian host.
Keywords: Trypanosoma cruzi
Chagas disease
keywords: Trypanosoma cruzi
Doença de Chagas
Issue Date: 2017
Publisher: Blackwell Publishing
Citation: KESSLER, Rafael Luis et al. Recently differentiated epimastigotes from Trypanosoma cruzi are infective to the mammalian host. Mol Microbiol., v. 104, n. 5, p. 712-736, 2017.
DOI: 10.1111/mmi.13653
ISSN: 0950-382X
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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