Genetic diversity of Mycobacterium tuberculosis from Pará, Brazil, reveals a higher frequency of ancestral strains than previously reported in South America

Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. WHO Supranational TB Reference Laboratory. Abymes, Guadalupe, France.
Institut Pasteur de Guadeloupe. Unité de la Tuberculose et des Mycobactéries. WHO Supranational TB Reference Laboratory. Abymes, Guadalupe, France.
Instituto Evandro Chagas. Ananindeua, Pará, Brasil.
Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada as Micobactérias. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada as Micobactérias. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada as Micobactérias. Rio de Janeiro, RJ. Brasil / Institute of Tropical Medicine. Mycobacterium Unit. Antwerp, Belgium.
Universidade Federal do Pará. Instituto de Ciências Biológicas. Laboratório de Polimorfismo de DNA. Belém, PA, Brasil.
Universidade Federal do Pará. Núcleo de Medicina Tropical. Belém, PA, Brasil.
Univ. Paris-Sud. Université Paris-Saclay. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS. France.
Instituto Evandro Chagas. Ananindeua, Pará, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Instituto Evandro Chagas. Ananindeua, Pará, Brasil / Universidade do Estado do Pará. Programa de Pós-graduação em Biologia Parasitária na Amazônia. Belém, PA, Brasil

Abstract

There is only scarce information available on genotypic diversity of the Mycobacterium tuberculosis complex (MTBC) clinical isolates circulating in the Northern part of Brazil, a relatively neglected region regarding research on tuberculosis. We therefore characterized 980 MTBC clinical isolates from the state of Pará, by spoligotyping and data was compared with patterns from around the world, besides analyzing drug susceptibility, and collecting sociodemographic data. We also performed 24 loci MIRU-VNTR typing to evaluate phylogenetic inferences among the East-African-Indian (EAI) lineage strains. The Geographic Information System analyses were performed to generate a descriptive visualization of MTBC strain distribution in the region. A total of 249 different spoligopatterns primarily belonging to evolutionary recent Euro-American lineages, as well as Central-Asian, Manu and ancestral EAI lineages, were identified, in addition to strains with reportedly unknown lineage signatures. The most frequent lineages were Latin American Mediterranean, T and Haarlem. Interestingly, EAI lineage strains were found in a significantly higher proportion in comparison with previous studies from South America. Regarding EAI lineage, the absence of spacers 4-9 and 23-24 co-related to 24 loci MIRU-VNTRs may suggest a close evolutionary relationship between such strains in Pará and those prevalent in Mozambique, which might have contributed to the genetic diversity of MTBC strains in this region.

Keywords in Portuguese

Mycobacterium tuberculosis
Estrutura Populacional
Brasil
Linhagem euro-americana

Keywords

Mycobacterium tuberculosis
Population-structure
Spoligotyping
Brazil
Euro-American lineage
EAI lineage

Publisher

Elsevier

Citation

CONCEIÇÃO, Emilyn Costa; et al. Genetic diversity of Mycobacterium tuberculosis from Pará, Brazil, reveals a higher frequency of ancestral strains than previously reported in South America. Infection, Genetic and Evolution, v.56, p.62-74, 2017.

DOI

10.1016/j.meegid.2017.10.021

ISSN

1567-1348

Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26830

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Restricted access

Embargo date

2030-01-01

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