Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26870
Title: First molecular characterization of Balantioides coli (Malmsten, 1857) isolates maintained in vitro culture and from feces of captive animals, Rio de Janeiro, Brazil
Authors: Barbosa, Alynne da Silva
Ponce-Gordo, Francisco
Dib, Lais Verdan
Uchôa, Claudia M. Antunes
Bastos, Otílio Machado Pereira
Pissinatti, Alcides
Amendoeira, Maria Regina Reis
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Setor de Parasitologia. Niterói, RJ, Brasil.
Universidad Complutense de Madrid. Facultad de Farmacia. Departamento de Parasitologia. Madrid, Spain.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Setor de Parasitologia. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Setor de Parasitologia. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Setor de Parasitologia. Niterói, RJ, Brasil.
Centro de Primatologia do Rio de Janeiro. INEA. Guapimirim, RJ, Brasil / Centro Universitário Serra dos Órgãos. Teresópolis, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil.
Abstract: Ciliate protozoa of the genus Balantioides can parasitize a variety of animals. The morphology of the evolutionary forms of the parasite and the host species affected have long been the only characteristics used to taxonomically identify the species of these protozoa, but these variables are not very precise. To confirm species identity, molecular biology tools are currently used. In this context, this study aimed to analyze protozoan isolates maintained in culture medium and from fecal samples from captive animals in Rio de Janeiro, Brazil, by means of molecular tools. Forty isolates maintained in Pavlova modified medium (30 were isolated from feces of pigs and 10 from feces of cynomolgus macaques) were analyzed. In addition, 34 fecal samples (8 from pigs, 8 from cynomolgus macaques and 18 from rhesus macaques) containing Balantioides coli-like cysts were analyzed. All samples were subjected to DNA extraction and the polymerase chain reaction (PCR) to amplify the fragment ITS1 - 5.8s rRNA - ITS2, and the PCR products were purified and sequenced. All samples (100%) presented sequences that were grouped in the Balantioides coli cluster. The type A0 variant predominated. These sequences were 96% to 99% identical to those deposited in GenBank, including a B. coli sequence that had been obtained from human fecal material in Bolivia. It seems that the culturing system did not select variants, because this variant was also seen in the amplified sequences of fecal samples containing cysts. The isolate sequences in the cultures showed few ambiguities and substitutions, thus generating reliable chromatograms. This was the first study to identify B. coli in captive animals in Brazil, through molecular biology. In addition, it was the first to evaluate a large panel of isolates of the parasite through culturing.
Keywords: Balantioides
Xenical culture
Molecular analysis
Brasil
keywords: Balantioides
Análise molecular
Cultura xenical
Brasil
Issue Date: 2017
Publisher: Elsevier
Citation: BARBOSA, Alynne da Silva; et al. First molecular characterization of Balantioides coli (Malmsten, 1857) isolates maintained in vitro culture and from feces of captive animals, Rio de Janeiro, Brazil. Veterinary Parasitology, Regional Studies and Reports, v.10, p.102-113, Dec. 2017.
DOI: 10.1016/j.vprsr.2017.08.014
ISSN: 2405-9390
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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