Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26877
Title: Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2
Authors: Matos, Natália Alves de
Silva, Josiane Fernandes da
Matsui, Tamires Cardoso
Damasceno, Karine Araujo
Duarte, Igor Dimitri Gama
Lemos, Virgínia Soares
Cassali, Geovanni D
Klein, André
Affilliation: Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofísica. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Patologia Geral. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Farmacologia. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Farmacologia. Belo Horizonte, MG, Brasil
Abstract: Proteinase-activated receptor (PAR) 2 has been implicated in eosinophil migration. Mast cell (MC) tryptase has been similarly implicated in allergic diseases through the activation of PAR-2, but the role of this receptor in MC tryptase-induced inflammation is not well elucidated. This study aims to investigate the ability of MC tryptase or PAR-2 activating peptide (SLIGRL-NH2) to induce eosinophil recruitment to the pleural cavity of mice. Mast cell tryptase-injected mice were pretreated with PAR-2 antagonist ENMD-1068. Mice injected with SLIGRL-NH2 were pretreated with mast cell tryptase inhibitor APC 366, and eosinophil migration into the pleural cavity and PAR-2 expression was analyzed after 24 or 48 h. SLIGRL-NH2-induced eosinophil recruitment was inhibited by APC 366, and MC tryptase-induced eosinophil recruitment was abolished by ENMD-1068. MC tryptase induced PAR-2 expression on pleural eosinophils. Our results demonstrate a key role for PAR-2 in mediating eosinophil recruitment in MC tryptase-induced pleurisy in mice. The ability of MC tryptase to inducing PAR-2 expression on eosinophils corroborates the relevance of MC tryptase and PAR-2 on modulating eosinophil migration.
Keywords: Eosinophils
Eosinophil migration
Mast cell tryptase
PAR-2
Pleurisy
keywords: Eosinófilos
Migração de eosinófilos
Triptase de mastócitos
PAR-2
Pleurisia
Issue Date: 2013
Publisher: Springer Verlag
Citation: Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2
Description: Damasceno, Karine Araújo. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
DOI: 10.1007/s10753-013-9664-5
ISSN: 0360-3997
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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