Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26882
Title: The midgut microbiota plays an essential role in sand fly vector competence for Leishmania major
Authors: Louradour, Isabelle
Monteiro, Carolina Cunha
Inbar, Ehud
Ghosh, Kashinath
Merkhofer, Richard
Lawye, Phillip
Paun, Andrea
Smelkinson, Margery
Secundino, Nagila Francinete Costa
Lewis, Michael
Erram, Dinesh
Zurek, Ludek
Sacks, David
Affilliation: Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Entomologia Médica. Belo Horizonte, MG, Brazil
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Biological Imaging Section. Research Technologies Branch. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Bethesda, MD, USA
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Entomologia Médica. Belo Horizonte, MG, Brazil
Department of Pathogen Molecular Biology. London School of Hygiene and Tropical Medicine. London, UK
Department of Entomology .Kansas State University. Manhattan, KS, USA
Department of Entomology .Kansas State University. Manhattan, KS, USA
Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institute of Health. Bethesda, MD, USA
Abstract: For many arthropod vectors, the diverse bacteria and fungi that inhabit the gut can negatively impact pathogen colonization. Our attempts to exploit antibiotic treatment of colonized Phlebotomus duboscqi sand flies in order to improve their vector competency for Leishmania major resulted instead in flies that were refractory to the development of transmissible infections due to the inability of the parasite to survive and to colonize the anterior midgut with infective, metacyclic stage promastigotes. The parasite survival and development defect could be overcome by feeding the flies on different symbiont bacteria but not by feeding them on bacterial supernatants or replete medium. The inhibitory effect of the dysbiosis was moderated by lowering the concentration of sucrose (<30% w/v) used in the sugar feeds to maintain the colony. Exposure of promastigotes to 30% sucrose was lethal to the parasite in vitro. Confocal imaging revealed that the killing in vivo was confined to promastigotes that had migrated to the anterior plug region, corresponding to the highest concentrations of sucrose. The data suggest that sucrose utilization by the microbiota is essential to promote the appropriate osmotic conditions required for the survival of infective stage promastigotes in vivo.
Keywords: Leishmania
microbiota
osmotic stress
sand flies
vector competence
keywords: Leishmania
microbiota
estresse osmótico
Issue Date: 2017
Publisher: Blackwell Publishing
Citation: LOURADOUR, Isabelle et al. The midgut microbiota plays an essential role in sand fly vector competence for Leishmania major. Cell Microbiol., v. 19, n. 10, e12755, 2017
DOI: 10.1111/cmi.12755
ISSN: 1462-5814
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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