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GENE EXPRESSION PROFILING DURING EARLY ACUTE FEBRILE STAGE OF DENGUE INFECTION CAN PREDICT THE DISEASE OUTCOME
Idoso, 80 anos ou mais
Apoptose
Biomarcadores / metabolismo
Brasil
Estudos de coorte
Dengue / diagnóstico
Dengue / genética
Dengue / metabolismo
Vírus da Dengue / genética
Vírus da Dengue / metabolismo
Fêmea
Febre / diagnóstico
Febre / metabolismo
Regulamento de Expressão Gênica
Humanos
Masculino
Meia idade
Análise multivariada
Análise de Sequência de Sequência de Oligonucleótidos
Resultado do tratamento
Author
Affilliation
Abstract
Background: We report the detailed development of biomarkers to predict the clinical outcome under dengue infection.
Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome geneexpression
microarray data, validated by quantitative PCR and tested in independent samples.
Methodology/Principal Findings: The study was performed on patients of a well-characterized dengue cohort from Recife,
Brazil. The samples analyzed were collected prospectively from acute febrile dengue patients who evolved with different
degrees of disease severity: classic dengue fever or dengue hemorrhagic fever (DHF) samples were compared with similar
samples from other non-dengue febrile illnesses. The DHF samples were collected 2–3 days before the presentation of the
plasma leakage symptoms. Differentially-expressed genes were selected by univariate statistical tests as well as multivariate
classification techniques. The results showed that at early stages of dengue infection, the genes involved in effector
mechanisms of innate immune response presented a weaker activation on patients who later developed hemorrhagic fever,
whereas the genes involved in apoptosis were expressed in higher levels.
Conclusions/Significance: Some of the gene expression signatures displayed estimated accuracy rates of more than 95%,
indicating that expression profiling with these signatures may provide a useful means of DHF prognosis at early stages of
infection.
DeCS
AdultoIdoso, 80 anos ou mais
Apoptose
Biomarcadores / metabolismo
Brasil
Estudos de coorte
Dengue / diagnóstico
Dengue / genética
Dengue / metabolismo
Vírus da Dengue / genética
Vírus da Dengue / metabolismo
Fêmea
Febre / diagnóstico
Febre / metabolismo
Regulamento de Expressão Gênica
Humanos
Masculino
Meia idade
Análise multivariada
Análise de Sequência de Sequência de Oligonucleótidos
Resultado do tratamento
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