Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26884
Title: Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome
Authors: Nascimento, Eduardo J. M.
Braga-Neto, Ulisses
Calzavara-Silva, Carlos E.
Gomes, Ana L. V.
Abath, Frederico G. C.
Brito, Carlos A. A.
Cordeiro, Marli T.
Silva, Ana M.
Magalhães, Cecilia
Andrade, Raoni
Gil, Laura H. V. G.
Marques, Ernesto T. A.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / The Johns Hopkins University School of Medicine. Division of Infectious Disease. Department of Medicine. Baltimore, Maryland, United States of America.
Texas A&M University. College Station. Department of Electrical Engineering. Texas, United States of America.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Universidade Federal de Pernambuco. Departamento de Medicina Clínica. Recife, PE, Brazil / Hospital Esperança. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil / Secretaria de Saúde do Estado de Pernambuco. Laboratório Central de Saúde Pública. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia e Terapia Experimental. Recife, PE, Brasil.
Abstract: Background: We report the detailed development of biomarkers to predict the clinical outcome under dengue infection. Transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome geneexpression microarray data, validated by quantitative PCR and tested in independent samples. Methodology/Principal Findings: The study was performed on patients of a well-characterized dengue cohort from Recife, Brazil. The samples analyzed were collected prospectively from acute febrile dengue patients who evolved with different degrees of disease severity: classic dengue fever or dengue hemorrhagic fever (DHF) samples were compared with similar samples from other non-dengue febrile illnesses. The DHF samples were collected 2–3 days before the presentation of the plasma leakage symptoms. Differentially-expressed genes were selected by univariate statistical tests as well as multivariate classification techniques. The results showed that at early stages of dengue infection, the genes involved in effector mechanisms of innate immune response presented a weaker activation on patients who later developed hemorrhagic fever, whereas the genes involved in apoptosis were expressed in higher levels. Conclusions/Significance: Some of the gene expression signatures displayed estimated accuracy rates of more than 95%, indicating that expression profiling with these signatures may provide a useful means of DHF prognosis at early stages of infection.
Keywords: Dengue
PCR
Acute febrile dengue
keywords: Dengue
PCR
Dengue febril aguda
DeCS: Adulto
Idoso, 80 anos ou mais
Apoptose
Biomarcadores / metabolismo
Brasil
Estudos de coorte
Dengue / diagnóstico
Dengue / genética
Dengue / metabolismo
Vírus da Dengue / genética
Vírus da Dengue / metabolismo
Fêmea
Febre / diagnóstico
Febre / metabolismo
Regulamento de Expressão Gênica
Humanos
Masculino
Meia idade
Análise multivariada
Análise de Sequência de Sequência de Oligonucleótidos
Resultado do tratamento
Issue Date: 2009
Citation: NASCIMENTO, E. J. M. et al. Gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome. PloS One, v. 4, n. 11, p. e7892, 19 nov. 2009.
DOI: 10.1371/journal.pone.0007892
ISSN: 1932-6203
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos

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