Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/26886
Title: IL-10 and TGF-β unbalanced levels in neutrophils contribute to increase inflammatory cytokine expression in childhood obesity
Authors: Medeiros, Nayara Ingrid de
Mattos, Rafael Teixeira de
Menezes, Carlos Alberto
Gusmão, Rafaelle Christine Gomes Fares
Talvani, André
Dutra, Walderez Ornelas
Santos, Fabricio Rios
Oliveira, Rodrigo Correa de
Estanislau, Juliana de Assis Silva Gomes
Affilliation: Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil
Universidade Estadual de Santa Cruz. Departamento de Genética. Ilhéus, BA, Brazil/Serviço de Medicina Preventiva da Unimed. Aracaju, SE, Brazil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/United States Food and Drug Administration. Center for Biologics Evaluation and Research. Silver Spring, MD, USA
Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Laboratório de Imunobiologia da Inflamação. Ouro Preto, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, Brazil.
Universidade Federal de Mato Grasso. Faculdade de Medicina. Departamento de Ciências Básicas da Saúde. Cuiabá, MT, Brazil.
Fundação Oswaldo Cruz. Instituto René Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/Universidade Federal de Ouro Preto. Departamento de Ciências Biológicas. Laboratório de Imunobiologia da Inflamação. Ouro Preto, MG, Brazil/Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Belo Horizonte, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, Brazil.
Abstract: PURPOSE: Obesity is a multifactorial disease, associated with metabolic disorders, chronic low-grade inflammation, and impaired immunity. This study aimed to evaluate the childhood obesity-associated effects on neutrophil activation and cytokine production. METHODS: We evaluated activation and recognition markers and cytokine production in neutrophils from the peripheral blood of children with obesity and normal weight using multicolor flow cytometry. RESULTS: We demonstrate a higher frequency of neutrophils in childhood obesity group (CO) compared to normal-weight group (NW). Our data showed that neutrophils from CO group are capable of antigen recognition and presentation through higher expression of TLR-4 (CD284) and HLA-DR in comparison with neutrophils from NW. On the other hand, neutrophils from CO group are faulty to deliver co-stimulatory signals, through lower expression of co-stimulatory molecules. We showed an increased expression of IL-6, IL-1β, IL-12, and TNF, and decreased expression of IL-8 and IL-10 by neutrophils from CO compared to NW, while TGF-β is equivalently expressed in neutrophils from both groups. Despite this, we observed that TGF-β/inflammatory cytokine ratio was significantly higher than the IL-10/inflammatory cytokine ratio only in CO group. Our analysis showed obesity altering the correlation profile for the expression of co-stimulatory, recognition, and activation molecules, as well as for cytokines by neutrophils, suggesting an association between lower IL-10 expression and inflammation in childhood obesity. CONCLUSIONS: The unbalance between the ratio of IL-10 and TGF-β expressions, the IL-10 lower expression, and changes in correlation profile seem to contribute with an inefficient regulation of inflammatory cytokine expression in childhood obesity. However, these changes still not may be considered the sole mechanism that directs inflammation during childhood obesity, once other molecules, pathways, and cells should be evaluated.
Keywords: Childhood obesity
Cytokines
Immune regulation
Innate immunity
Neutrophils
keywords: Obesidade infantil
citocinas
Regulação imune
Imunidade inata
Neutrófilos
Issue Date: 2017
Publisher: Springer Verlag
Citation: MEDEIROS, Nayara Ingrid de et al. IL-10 and TGF-β unbalanced levels in neutrophils contribute to increase inflammatory cytokine expression in childhood obesity. Eur J Nutr., 2017
DOI: 10.1007/s00394-017-1515-y
ISSN: 1436-6207
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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