| Author | Lopes, Marcelo Donizete | |
| Author | Oliveira, Flavio Martins | |
| Author | Coelho, Ivan Evangelista V. | |
| Author | Passos, Maria Juliana Ferreira | |
| Author | Alves, Clarice Carvalho | |
| Author | Taranto, Alex Gutterres | |
| Author | Junior, Moacyr Comar | |
| Author | Santos, Luciana Lara dos | |
| Author | Fonseca, Cristina Toscano | |
| Author | Perez, José Augusto Ferreira | |
| Author | Lopes, Débora de Oliveira | |
| Access date | 2018-06-15T12:46:36Z | |
| Available date | 2018-06-15T12:46:36Z | |
| Document date | 2017 | |
| Citation | LOPES, Marcelo Donizete et al. Epitopes rationally selected through computational analyses induce T-cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni. Biotechnol Prog. , v. 33, n. 3, p. 804-814, 2017. | pt_BR |
| ISSN | 8756-7938 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/26903 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Schistosoma mansoni | pt_BR |
| Subject in Portuguese | bioinformatica | pt_BR |
| Subject in Portuguese | diagnostico | pt_BR |
| Subject in Portuguese | alvos de vacina | pt_BR |
| Subject in Portuguese | modelagem molecular | pt_BR |
| Title | Epitopes rationally selected through computational analyses induce T-cell proliferation in mice and are recognized by serum from individuals infected with Schistosoma mansoni. | pt_BR |
| Type | Article | |
| DOI | 10.1002/btpr.2463 | |
| Abstract | Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis. The three-dimensional structure of selected epitopes was constructed and used in molecular docking to verify the interaction with murine MHCII H2-IA(b) . In this process, four epitopes were selected and synthesized to assess their ability to stimulate proliferation of CD4(+) T lymphocytes in mice splenocyte cultures. The results showed that Sm041370 and Sm168240 epitopes induced significant cell proliferation. Additionally, the four epitopes were used as antigens in the Indirect Enzyme-Linked Immunosorbent Assay (ELISA) to assess the recognition by serum from individuals infected with Schistosoma mansoni. Sm140560, Sm168240 and Sm041370 epitopes were recognized by infected individuals IgG antibodies. Therefore, Sm041370 and Sm168240 epitopes that stood out in in silico and in vitro analyses could be promising antigens in schistosomiasis vaccine development or diagnostic kits. | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | bioinformatics | pt_BR |
| Subject | diagnosis | pt_BR |
| Subject | vaccine targets | pt_BR |
| Subject | molecular modeling | pt_BR |
| Embargo date | 2080-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
