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2080-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
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EPITOPES RATIONALLY SELECTED THROUGH COMPUTATIONAL ANALYSES INDUCE T-CELL PROLIFERATION IN MICE AND ARE RECOGNIZED BY SERUM FROM INDIVIDUALS INFECTED WITH SCHISTOSOMA MANSONI.
bioinformatica
diagnostico
alvos de vacina
modelagem molecular
Author
Affilliation
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil
Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil
Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Fundacao Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Sıntese Organica e Nanoestruturas Divinopolis, MG, Brasil
Universidade Federal de Sao Joao Del Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Abstract
Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis. The three-dimensional structure of selected epitopes was constructed and used in molecular docking to verify the interaction with murine MHCII H2-IA(b) . In this process, four epitopes were selected and synthesized to assess their ability to stimulate proliferation of CD4(+) T lymphocytes in mice splenocyte cultures. The results showed that Sm041370 and Sm168240 epitopes induced significant cell proliferation. Additionally, the four epitopes were used as antigens in the Indirect Enzyme-Linked Immunosorbent Assay (ELISA) to assess the recognition by serum from individuals infected with Schistosoma mansoni. Sm140560, Sm168240 and Sm041370 epitopes were recognized by infected individuals IgG antibodies. Therefore, Sm041370 and Sm168240 epitopes that stood out in in silico and in vitro analyses could be promising antigens in schistosomiasis vaccine development or diagnostic kits.
Keywords in Portuguese
Schistosoma mansonibioinformatica
diagnostico
alvos de vacina
modelagem molecular
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