Cuticular hydrocarbons (CHs) contain complex mixtures of components that cover the surface of all insects to restrict water loss, and in many species function in chemical communication. The complex mixtures have been used as a chemotaxonomic tool since the 1970s. The majority of CH components can be identified by gas chromatography-mass spectrometry (GC-MS), allowing hydrocarbon profiles to be used as chemotaxonomic characters, which can differentiate insect species, including cryptic species. The present study used GC-MS for the identification of CHs of males and females of three species of calliphorids: Cochliomyia macellaria (Fabricius), Hemilucilia segmentaria (Fabricius) and Lucilia cuprina (Wiedmann), A comparison of the chromatographic profiles from the three species allows them to be readily separated. Hydrocarbons identified from the three species contain 23 to 37 carbons and include n-alkanes, methyl-branched alkanes and alkenes. The most abundant compound for both sexes of C. macellaria was n-heptacosane, while for H. segmentaria it was 2-methyltriacontane; for L. cuprina, however, the most abundant compound was hentriacontene for females and nonacosene for males. The results obtained in this study are important for the establishment of a database to be used in chemotaxonomy of sanitarily and forensically important insects.