Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27047
Title: Milonine, an Alkaloid of Cissampelos sympodialis Eichl. (Menispermaceae) Inhibits Histamine Release of Activated Mast Cells
Authors: Alves, Adriano Francisco
Vieira, Giciane Carvalho
Gadelha, Francisco Allysson A. F.
Silva, Luiz Henrique Agra Cavalcante
Martins, Marco Aurélio
Barbosa Filho, José Maria
Piuvezam, Marcia Regina
Affilliation: Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil / Universidade Federal da Paraíba. Centro de Ciências da Saúde. Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ. Brasil.
Universidade Federal da Paraíba. Centro de Ciências da Saúde. Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos. João Pessoa, PB, Brasil.
Universidade Federal da Paraíba. Centro de Ciências da Saúde. Laboratório de Imunofarmacologia. João Pessoa, PB, Brasil / Universidade Federal da Paraíba. Centro de Ciências da Saúde. Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos. João Pessoa, PB, Brasil.
Abstract: Milonine is an alkaloid of Cissampelos sympodialis Eichl. (Menispermaceae), a plant used in the northeast of Brazil to treat allergies such as asthma, rhinitis, and other conditions. Previously, several alkaloids were isolated from its roots and leaves with pharmacological properties in asthma and acute inflammation models. Therefore, the aim of this study was to evaluate the milonine effect on mast cells degranulation in vivo and in vitro. Swiss mice (n = 8) were used in models of paw edema induced by carrageenan, compound 48/80, or histamine. One hour before challenge, the animals were treated with milonine (at different doses) or standard drugs and, at different time points, the edema formation was measured. In addition, other different methods, such as anaphylactic shock reaction and scratching behavior models both induced by compound 48/80, a mast cell degranulator, were used to assess milonine effect histamine release in vivo. Moreover, milonine effect on mast cell degranulation in vitro was also carried out. Firstly, it was observed that milonine significantly decreased the carrageenan edema formation only at the beginning of the reaction (i.e., up to 2 h after challenge). Furthermore, this alkaloid decreased the edema induced by compound 48/80, maintained the paw tissue integrity, without modulating histamine-induced paw edema. In anaphylactic shock reaction, milonine increased the time of animal survival when compared with compound 48/80 group. Milonine also significantly decreased the scratching behavior induced by compound 48/80 with decreasing of mast cell degranulation in vitro. Therefore, these data indicated that milonine presents anti-allergic properties by decreasing mast cell degranulation rather than acting on histamine effect.
Keywords: milonine
mast cell
degranulation
histamine
alkaloid
Cissampelos sympodialis Eichl
(Menispermaceae)
keywords: Mastócitos
Degranulação
Histamina
Alcaloides
Menispermaceae
Issue Date: 2017
Publisher: Springer Verlag (Germany)
Citation: ALVES, Adriano Francisco; et al. Milonine, an Alkaloid of Cissampelos sympodialis Eichl. (Menispermaceae) Inhibits Histamine Release of Activated Mast Cells. Inflammation, v.40, n.6, 11p, Dec.2017.
DOI: 10.1007/s10753-017-0652-z
ISSN: 0360-3997
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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