Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27058
Title: Epitopes rationally selected through computational analyses induce T-cell proliferation in miceand are recognized by serum from individuals infected with Schistosoma mansoni
Authors: Lopes, Marcelo D.
Oliveira, Flavio M.
Coelho, Ivan E. V.
Passos, Maria J. F.
Alves, Clarice Carvalho
Taranto, Alex G.
Junior, Moacyr Comar
Santos, Luciana L.
Fonseca, Cristina Toscano
Villar, Jose A. F. P.
Lopes, Debora O.
Affilliation: Universidade Federal de Sao Joao del-Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Sıntese Organica e Nanoestruturas.. Divinopolis, MG, Brasil
Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Quımica Farmaceutica. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Grupo de Pesquisa em Biologia Parasitaria e Imunologia. Fundação Oswaldo Cruz. Instituto Rene Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Sıntese Organica e Nanoestruturas. Divinopolis, MG, Brasil
Universidade Federal de Sao Joao del-Rei. Laboratorio de Biologia Molecular. Divinopolis, MG, Brasil
Abstract: Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis. The three-dimensional structure of selected epitopes was constructed and used in molecular docking to verify the interaction with murine MHCII H2-IAb. In this process, four epitopes were selected and synthesized to assess their ability to stimulate proliferation of CD41 T lymphocytes in mice splenocyte cultures. The results showed that Sm041370 and Sm168240 epitopes induced significant cell proliferation. Additionally, the four epitopes were used as antigens in the Indirect Enzyme-Linked Immunosorbent Assay (ELISA) to assess the recognition by serum from individuals infected with Schistosoma mansoni. Sm140560, Sm168240, and Sm041370 epitopes were recognized by infected individuals IgG antibodies. Therefore, Sm041370 and Sm168240 epitopes that stood out in in silico and in vitro analyses could be promising antigens in schistosomiasis vaccine development or diagnostic kits.
Keywords: Schistosoma mansoni
bioinformatics
diagnosis
vaccine targets
molecular modeling
keywords: Schistosoma mansoni
bioinformatica
diagnostico
Issue Date: 2017
Publisher: Wiley Online Library
Citation: LOPES, Marcelo D. et al. Epitopes rationally selected through computational analyses induce T-cell proliferation in miceand are recognized by serum from individuals infected with Schistosoma mansoni . Biotechnology Progress, v. 33, p. 804-814, 2017.
DOI: 10.1002/btpr.2463
ISSN: 8756-7938
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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