Author | Rizvi, Farrukh S | |
Author | Afchain, Daniel I | |
Author | Sherlock, Ítalo Rodrigues de Araújo | |
Author | Sadigursky, Moysés | |
Author | Capron, André | |
Author | Santoro, Ferrucio | |
Access date | 2018-06-25T14:31:31Z | |
Available date | 2018-06-25T14:31:31Z | |
Document date | 1985 | |
Citation | RIZVI, F. S. et al. Infectivity of Leishmania promastigotes is associated with surface antigenic expression. Immunology Letters, v. 11, p. 317-323, 1985. | pt_BR |
ISSN | 0165-2478 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/27079 | |
Sponsorship | The Rockefeller Foundation, UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases and EEC (grant no. TSD-M-010-F(RS). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Interações macrofágicas | pt_BR |
Subject in Portuguese | Infectividade | pt_BR |
Subject in Portuguese | Expressão antigênica de superfície | pt_BR |
Subject in Portuguese | Anticorpos policlonais | pt_BR |
Title | Infectivity of Leishmania promastigotes is associated with surface antigenic expression | pt_BR |
Type | Article | pt_BR |
Abstract | Differentiation between a non-infective and an
infective Leishrnania promastigote population
was demonstrated. Promastigotes in the stationary
phase (day 5) were found to be highly infective
in vitro to BALB/c mouse peritoneal macrophages,
compared with those of the logarithmic
phase (day 3). The infective promastigotes
showed surface antigenic determinants different
from non-infective ones. Polyclonal anti-3 day
and anti-5 day antibodies were bound specifically
to the surface of corresponding promastigotes in
both SRIA and IFAT; no strong cross-reactions
were observed otherwise. Also, polyclonal
anti-5 day but not anti-3 day antibodies recognized
efficiently the antigenic molecules on the
surface of late stage (day 7) sandfly
promastigotes. This clearly indicates the appearance
of new antigenic molecules on the surface
of infective promastigote forms. Intracellular multiplication of Leishmania was significantly inhibited
by anti-5 day antibodies compared with
anti-3 day antibodies. The presence of new surface
molecules on late stage promastigotes may
contribute to Leishmania infectivity. | pt_BR |
Affilliation | Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France | pt_BR |
Affilliation | Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France | pt_BR |
Affilliation | Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Macrophage interactions | pt_BR |
Subject | Infectivity | pt_BR |
Subject | Surface antigenic expression | pt_BR |
Subject | Polyclonal antibodies | pt_BR |