Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27079
Title: Infectivity of Leishmania promastigotes is associated with surface antigenic expression
Authors: Rizvi, Farrukh S
Afchain, Daniel I
Sherlock, Ítalo Rodrigues de Araújo
Sadigursky, Moysés
Capron, André
Santoro, Ferrucio
Affilliation: Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France
Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France
Institut Pasteur. Centre d'Immunologie et de Biologie Parasitaire. Lille Cedex, France
Abstract: Differentiation between a non-infective and an infective Leishrnania promastigote population was demonstrated. Promastigotes in the stationary phase (day 5) were found to be highly infective in vitro to BALB/c mouse peritoneal macrophages, compared with those of the logarithmic phase (day 3). The infective promastigotes showed surface antigenic determinants different from non-infective ones. Polyclonal anti-3 day and anti-5 day antibodies were bound specifically to the surface of corresponding promastigotes in both SRIA and IFAT; no strong cross-reactions were observed otherwise. Also, polyclonal anti-5 day but not anti-3 day antibodies recognized efficiently the antigenic molecules on the surface of late stage (day 7) sandfly promastigotes. This clearly indicates the appearance of new antigenic molecules on the surface of infective promastigote forms. Intracellular multiplication of Leishmania was significantly inhibited by anti-5 day antibodies compared with anti-3 day antibodies. The presence of new surface molecules on late stage promastigotes may contribute to Leishmania infectivity.
Keywords: Leishmania
Macrophage interactions
Infectivity
Surface antigenic expression
Polyclonal antibodies
keywords: Leishmania
Interações macrofágicas
Infectividade
Expressão antigênica de superfície
Anticorpos policlonais
Issue Date: 1985
Publisher: Elsevier
Citation: RIZVI, F. S. et al. Infectivity of Leishmania promastigotes is associated with surface antigenic expression. Immunology Letters, v. 11, p. 317-323, 1985.
ISSN: 0165-2478
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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