Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27141
Title: BbrzSP-32, the first serine protease isolated fromBothrops brazili venom: Purification and characterization.
Authors: Zaqueo, Kayena D.
Kayano, Anderson M.
Domingos, Thaisa F. S.
Moura, Laura A.
Fuly, André L.
Silva, Saulo L. da.
Acosta., Gerardo.
Oliveira, Eliandre.
Albericio, Fernando.
Zanchi, Fernando B.
Zuliani, Juliana P.
Calderon, Leonardo A.
Stábeli, Rodrigo G.
Soares, Andreimar M.
Affilliation: Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil. / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Universidade Federal Fluminense. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Niterói, RJ, Brasil.
Universidade Federal Fluminense. Niterói, RJ, Brasil.
Universidade Regional Amazónica IKIAM. Tena, Napo, Ecuador.
Institute for Research in Biomedicine. Barcelona Science Park. Barcelona, Spain.
Barcelona Science Park. Proteomic Platform. Barcelona, Spain.
Institute for Research in Biomedicine. Barcelona Science Park. Barcelona, Spain / University of Barcelona. Department of Organic Chemistry. Barcelona, Spain / University of KwaZulu Natal. School of Chemistry. Durban, South Africa.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Porto Velho, RO, Brasil / Universidade Federal de Rondônia. Departamento de Medicina. Porto Velho, RO, Brasil.
Abstract: Snake venom toxins are related not only in detention, death and the promotion of initial digestion of prey but also due to their different biochemical, structural and pharmacological effects they can result in new drugs. Among these toxins snake venom serine proteases (SVSPs) should be highlighted because they are responsible for inducing changes in physiological functions such as blood coagulation, fibrinolysis, and platelet aggregation. This article presents the first serine protease (SP) isolated from Bothrops brazili: BbrzSP-32. The new SP showed 36 kDa of relative molecular mass and its absolute mass was confirmed by mass spectrometry as 32,520 Da. It presents 79.48% identity when compared to other SVSPs and was able to degrade the α-chain of fibrinogen, in vitro models, because of this it is considered a SVTLE-A. It showed dose-dependent activity in the process of degradation of fibrin networks demonstrating greater specificity for this activity when compared to its thrombolytic action. BbrzSP-32 demonstrated proteolytic activity on gelatin and chromogenic substrates for serine proteases and thrombin-like enzymes (S-2288 and S-2238 respectively), besides having coagulant activity on human plasma. After pre-incubation with PMSF and benzamidine the coagulant and proteolytic activities on the S-2288 and S-2238 substrates were reduced. BbrzSP-32 shows stability against pH and temperature variations, demonstrating optimum activity between 30 and 40 °C and in the pH range 7.5 to 8.5. A new SP with potential biotechnological application was isolated.
Keywords: Snake venom
Enzyme
Biotechnology
Viperidae
Thrombin-like
Issue Date: 2016
Publisher: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology.
Citation: SOARES, ANDREIMAR M. et al. BbrzSP-32, the first serine protease isolated from Bothrops brazili venom: Purification and characterization. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, p. 15-25, 2016.
DOI: 10.1016/j.cbpa.2016.01.021
ISSN: 1095-6433
Copyright: restricted access
Appears in Collections:RO - Artigos de Periódicos




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