Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27508
Title: The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood
Authors: Cavalcanti, Milena de Paiva
Brito, Maria Edileuza Felinto de
Souza, Wayner Vieira de
Gomes, Yara de Miranda
Abath, Frederico G. C.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Coletiva. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: Recent research has demonstrated the high sensitivity of real time PCR (qPCR) in the diagnosis of Leishmania infantum infection. The goal of this study was to develop and evaluate a qPCR detection system for the diagnosis of visceral leishmaniosis (VL) in dogs. Specific primer sets were developed for the Leishmania donovani complex, in which a fragment of 132 bp of kDNA from L. infantum was amplified. The reaction was performed using the ABI PRISM 7000 system with ABI PRISM software used to carry out the analysis. When canine blood samples were assessed using this system the detection limit of the method was found to be 0.07 parasites per reaction, the efficiency was 94.17% (R(2)=0.93, slope=-3.47) and the sensitivity and specificity were 100% and 83.33% respectively. The use of such a sensitive, reproducible and rapid qPCR-based assay will be useful in the diagnosis and control of L. infantum infection in endemic areas, where serological surveys often underestimate true disease prevalence.
Keywords: Visceral leishmaniosis
Leishmania infantum
Real-time PCR (qPCR)
Diagnosis
keywords: Leishmaniose visceral
Leishmania infantum
PCR em tempo real (qPCR)
Diagnóstico
DeCS: Animais
DNA, cinetoplasto / sangue
DNA, cinetoplasto / genética
Doenças do Cão / diagnóstico
Doenças do Cão / parasitologia
Cães
Leishmania infantum / genética
Leishmania infantum / isolamento & purificação
Leishmaniose Visceral / sangue
Leishmaniose Visceral / parasitologia
Leishmaniose Visceral / veterinária
Reação em Cadeia da Polimerase / métodos
Reação em Cadeia da Polimerase / veterinária
Reprodutibilidade dos Resultados
Sensibilidade e Especificidade
Issue Date: 2009
Publisher: Elsevier
Citation: DE PAIVA CAVALCANTI, M. et al. The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood. Veterinary Journal (London, England: 1997), v. 182, n. 2, p. 356–358, nov. 2009.
DOI: 10.1016/j.tvjl.2008.05.018
ISSN: 1532-2971
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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