Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27510
Title: Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel
Authors: Santos, Luciane Amorim
Adhikarla, Haritha
Yan, Xiting
Wang, Zheng
Fouts, Derrick E
Vinetz, Joseph M
Alcantara, Luiz Carlos Junior
Hartskeerl, Rudy A
Goris, Marga G. A
Picardeau, Mathieu
Reis, Mitermayer Galvão dos
Townsend, Jeffrey P
Zhao, Hongyu
Ko, Albert Icksang
Wunder Junior, Elsio Augusto
Affilliation: Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
J. Craig Venter Institute. Rockville, MD, United States
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Institut Pasteur. ,Biology of Spirochetes Unit. Paris, France
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Leptospirosis is a worldwide zoonosis, responsible for more than 1 million cases and 60,000 deaths every year. Among the 13 pathogenic species of the genus Leptospira, serovars belonging to L. interrogans serogroup Icterohaemorrhagiae are considered to be the most virulent strains, and responsible for majority of the reported severe cases. Serovars Copenhageni and Icterohaemorrhagiae are major representatives of this serogroup and despite their public health relevance, little is known regarding the genetic differences between these two serovars. In this study, we analyzed the genome sequences of 67 isolates belonging to L. interrogans serovars Copenhageni and Icterohaemorrhagiae to investigate the influence of spatial and temporal variations on DNA sequence diversity. Out of the 1072 SNPs identified, 276 were in non-coding regions and 796 in coding regions. Indel analyses identified 258 indels, out of which 191 were found in coding regions and 67 in non-coding regions. Our phylogenetic analyses based on SNP dataset revealed that both serovars are closely related but showed distinct spatial clustering. However, likelihood ratio test of the indel data statistically confirmed the presence of a frameshift mutation within a homopolymeric tract of lic12008 gene (related to LPS biosynthesis) in all the L. interrogans serovar Icterohaemorrhagiae strains but not in the Copenhageni strains. Therefore, this internal indel identified can genetically distinguish L. interrogans serovar Copenhageni from serovar Icterohaemorrhagiae with high discriminatory power. To our knowledge, this is the first study to identify global sequence variations (SNPs and Indels) in L. interrogans serovars Copenhageni and Icterohaemorrhagiae.
Keywords: Leptospira
Leptospirosis
Copenhageni
Icterohaemorrhagiae
Whole-genome sequencing
SNPs
Indels
Phylogeny
keywords: Leptospira
Leptospirose
Copenhageni
Icterohaemorrhagiae
Sequenciamento do genoma completo
SNPs
Indels
Filogenia
Issue Date: 2018
Publisher: Frontiers Media
Citation: SANTOS, L. A. et al. Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel. Frontiers in Cellular and Infection Microbiology, v. 8, art. 193, 2018.
ISSN: 2235-2988
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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