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GENOMIC COMPARISON AMONG GLOBAL ISOLATES OF L. INTERROGANS SEROVARS COPENHAGENI AND ICTEROHAEMORRHAGIAE IDENTIFIED NATURAL GENETIC VARIATION CAUSED BY AN INDEL
Leptospirose
Copenhageni
Icterohaemorrhagiae
Sequenciamento do genoma completo
SNPs
Indels
Filogenia
Leptospirosis
Copenhageni
Icterohaemorrhagiae
Whole-genome sequencing
SNPs
Indels
Phylogeny
Author
Affilliation
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
J. Craig Venter Institute. Rockville, MD, United States
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Institut Pasteur. ,Biology of Spirochetes Unit. Paris, France
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
J. Craig Venter Institute. Rockville, MD, United States
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Royal Tropical Institute. KIT Biomedical Research. Amsterdam, Netherlands
Institut Pasteur. ,Biology of Spirochetes Unit. Paris, France
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Biostatistics. New Haven, CT, United States
Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
Abstract
Leptospirosis is a worldwide zoonosis, responsible for more than 1 million cases and
60,000 deaths every year. Among the 13 pathogenic species of the genus Leptospira,
serovars belonging to L. interrogans serogroup Icterohaemorrhagiae are considered
to be the most virulent strains, and responsible for majority of the reported severe
cases. Serovars Copenhageni and Icterohaemorrhagiae are major representatives of
this serogroup and despite their public health relevance, little is known regarding
the genetic differences between these two serovars. In this study, we analyzed the
genome sequences of 67 isolates belonging to L. interrogans serovars Copenhageni
and Icterohaemorrhagiae to investigate the influence of spatial and temporal variations
on DNA sequence diversity. Out of the 1072 SNPs identified, 276 were in non-coding
regions and 796 in coding regions. Indel analyses identified 258 indels, out of which 191
were found in coding regions and 67 in non-coding regions. Our phylogenetic analyses
based on SNP dataset revealed that both serovars are closely related but showed distinct
spatial clustering. However, likelihood ratio test of the indel data statistically confirmed
the presence of a frameshift mutation within a homopolymeric tract of lic12008 gene
(related to LPS biosynthesis) in all the L. interrogans serovar Icterohaemorrhagiae strains
but not in the Copenhageni strains. Therefore, this internal indel identified can genetically
distinguish L. interrogans serovar Copenhageni from serovar Icterohaemorrhagiae with
high discriminatory power. To our knowledge, this is the first study to identify global
sequence variations (SNPs and Indels) in L. interrogans serovars Copenhageni and
Icterohaemorrhagiae.
Keywords in Portuguese
LeptospiraLeptospirose
Copenhageni
Icterohaemorrhagiae
Sequenciamento do genoma completo
SNPs
Indels
Filogenia
Keywords
LeptospiraLeptospirosis
Copenhageni
Icterohaemorrhagiae
Whole-genome sequencing
SNPs
Indels
Phylogeny
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