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Title: Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
Authors: Chagas, Andrezza Campos
Medeiros, Jansen Fernandes
Astolfi Filho, Spartaco
Py-Daniel, Victor
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Coordenação de Pesquisas em Ciências da Saúde. Porto Velho, RO, Brasil.
Universidade Federal do Amazonas. Centro de Apoio Multidisciplinar. Laboratório de Tecnologias de DNA. Manaus, AM, Brasil.
Instituto Nacional de Pesquisas da Amazônia. Núcleo de Ciências Humanas e Sociais. Manaus, AM, Brasil.
Abstract: In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 μg ± 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ages analyzed (12 h, 24 h, 48 h and 72 h following emergence). Coagulation bioassays showed that the SGHs had activities that interacted at all levels of coagulation (the intrinsic, extrinsic and common pathways), by extending the plasma recalcification time, prothrombin time, thrombin time. This is the first report on the activity of salivary gland proteins from the main vector of onchocerciasis in Brazil. We also suggest detailed studies on the morphology and function of the salivary glands in order to understand the role of these proteins in host/vector interactions.
Keywords: Moscas negras
Glândulas salivares
Keywords in spanish: Black flies
Salivary glands
Issue Date: 2010
Publisher: Fundação Oswaldo Cruz.
Citation: CHAGAS, Andrezza Campos et al. Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 105, n. 2, p.174-178, Feb. 2010.
DOI: 10.1590/S0074-02762010000200011
ISSN: 0074-0276
Copyright: open access
Appears in Collections:RO - Artigos de Periódicos
MG - IRR - Artigos de Periódicos

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