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EXTERNAL QUALITY ASSESSMENT FOR ZIKA VIRUS MOLECULAR DIAGNOSTIC TESTING, BRAZIL
Vírus
Diagnósticos
Vigilância
RT-PCR em tempo real
Infecções transmitidas por vetores
Américas
Brasil
Viruses
Diagnostics
Surveillance
Real-time RT-PCR
Vector-borne infections
Americas
Brazil
Sensibilidade e especificidade
Zika virus / imunologia
Zika virus / crescimento & desenvolvimento
Zika virus / infecção
Zika virus / diagnóstico
Humanos
PCR em tempo real
Author
Fischer, Carlo
Pedroso, Celia
Mendrone, Alfredo
Filippis, Ana Maria Bispo de
Vallinoto, Antonio Carlos Rosário
Ribeiro, Bergmann Morais
Durigon, Edison Luiz
Marques, Ernesto T. A.
Campos, Gubio S.
Viana, Isabelle F. T.
Levi, José Eduardo
Scarpelli, Luciano Cesar
Nogueira, Mauricio Lacerda
Bastos, Michele de Souza
Souza, Nathalia C. Santiago
Khouri, Ricardo
Lira, Sanny
Komninakis, Shirley Vasconcelos
Baronti, Cécile
Charrel, Rémi N.
Kümmerer, Beate M.
Drosten, Christian
Brites, Carlos
Lamballerie, Xavier de
Niedrig, Matthias
Martins Netto, Eduardo
Drexler, Jan Felix
Pedroso, Celia
Mendrone, Alfredo
Filippis, Ana Maria Bispo de
Vallinoto, Antonio Carlos Rosário
Ribeiro, Bergmann Morais
Durigon, Edison Luiz
Marques, Ernesto T. A.
Campos, Gubio S.
Viana, Isabelle F. T.
Levi, José Eduardo
Scarpelli, Luciano Cesar
Nogueira, Mauricio Lacerda
Bastos, Michele de Souza
Souza, Nathalia C. Santiago
Khouri, Ricardo
Lira, Sanny
Komninakis, Shirley Vasconcelos
Baronti, Cécile
Charrel, Rémi N.
Kümmerer, Beate M.
Drosten, Christian
Brites, Carlos
Lamballerie, Xavier de
Niedrig, Matthias
Martins Netto, Eduardo
Drexler, Jan Felix
Affilliation
German Centre for Infection Research. Associated partner Charité–Universitätsmedizin. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
Fundação Pro-Sangue/Hemocentro de São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.
Federal University of Para. Belém, PA, Brazil.
University of Brasília. Brasília, DF, Brazil.
University of São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Pernambuco, PE, Brazil.
Federal University of Bahia. Salvador, BA, Brasil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Pernambuco, PE, Brazil.
Fundação Pro-Sangue/Hemocentro de São Paulo, São Paulo, SP, Brazil / University of São Paulo. São Paulo, SP, Brazil / Diagnósticos da América—DASA. São Paulo, SP, Brazil / Hospital Israelita Albert Einstein. São Paulo, SP, Brazil.
Diagnósticos da América—DASA. São Paulo, SP, Brazil.
Faculdade de Medicina de São José do Rio Preto. São José do Rio Preto, SP, Brazil.
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
University of São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Gonçalo Muniz Institute. Salvador, BA, Brazil.
Hospital Israelita Albert Einstein. São Paulo, SP, Brazil.
Federal University of São Paulo. São Paulo, SP, Brazil.
Aix Marseille Université. Marseille, France / Assistance Publique-Hopitaux Marseille. Marseille, France.
Aix Marseille Université. Marseille, France / Assistance Publique-Hopitaux Marseille. Marseille, France.
University of Bonn Medical Centre. Bonn, Germany.
German Centre for Infection Research. Associated partner Charité–Universitätsmedizin. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
Assistance Publique-Hopitaux Marseille. Marseille, France / Aix Marseille Université. Marseille, France.
Robert Koch Institute. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
German Centre for Infection Research. Associated partner Charité–Universitätsmedizin. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
Fundação Pro-Sangue/Hemocentro de São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.
Federal University of Para. Belém, PA, Brazil.
University of Brasília. Brasília, DF, Brazil.
University of São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Pernambuco, PE, Brazil.
Federal University of Bahia. Salvador, BA, Brasil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Pernambuco, PE, Brazil.
Fundação Pro-Sangue/Hemocentro de São Paulo, São Paulo, SP, Brazil / University of São Paulo. São Paulo, SP, Brazil / Diagnósticos da América—DASA. São Paulo, SP, Brazil / Hospital Israelita Albert Einstein. São Paulo, SP, Brazil.
Diagnósticos da América—DASA. São Paulo, SP, Brazil.
Faculdade de Medicina de São José do Rio Preto. São José do Rio Preto, SP, Brazil.
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
University of São Paulo. São Paulo, SP, Brazil.
Oswaldo Cruz Foundation. Gonçalo Muniz Institute. Salvador, BA, Brazil.
Hospital Israelita Albert Einstein. São Paulo, SP, Brazil.
Federal University of São Paulo. São Paulo, SP, Brazil.
Aix Marseille Université. Marseille, France / Assistance Publique-Hopitaux Marseille. Marseille, France.
Aix Marseille Université. Marseille, France / Assistance Publique-Hopitaux Marseille. Marseille, France.
University of Bonn Medical Centre. Bonn, Germany.
German Centre for Infection Research. Associated partner Charité–Universitätsmedizin. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
Assistance Publique-Hopitaux Marseille. Marseille, France / Aix Marseille Université. Marseille, France.
Robert Koch Institute. Berlin, Germany.
LAPI. Hospital Universitário Professor Edgard Santos. Salvador, BA, Brazil.
German Centre for Infection Research. Associated partner Charité–Universitätsmedizin. Berlin, Germany.
Abstract
We conducted an external quality assessment of Zika virus molecular diagnostic tests in Brazil using a new Zika virus standard. Of 15 laboratories, 73% showed limited sensitivity and specificity. Viral load estimates varied significantly. Continuous quality assurance is needed to adequately estimate risk for Zika virus-associated disease and determine patient care.
Keywords in Portuguese
Vírus ZikaVírus
Diagnósticos
Vigilância
RT-PCR em tempo real
Infecções transmitidas por vetores
Américas
Brasil
Keywords
Zika virusViruses
Diagnostics
Surveillance
Real-time RT-PCR
Vector-borne infections
Americas
Brazil
DeCS
Brasil / epidemiologiaSensibilidade e especificidade
Zika virus / imunologia
Zika virus / crescimento & desenvolvimento
Zika virus / infecção
Zika virus / diagnóstico
Humanos
PCR em tempo real
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