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RAPID, NONINVASIVE DETECTION OF ZIKA VIRUS IN AEDES AEGYPTI MOSQUITOES BY NEAR-INFRARED SPECTROSCOPY
Fernandes, Jill N. et al. | Date Issued: 2018
Author
Fernandes, Jill N.
Santos, Lilha M. B. dos
Carneiro, Thaís Chouin
Pavan, Márcio G.
Garcia, Gabriela A.
David, Mariana R.
Beier, John C.
Dowell, Floyd E.
Freitas, Rafael Maciel de
Sikulu-Lord, Maggy T.
Affilliation
University of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.
University of Miami Miller School of Medicine. Department of Public Health Services. Miami, FL, USA.
U.S. Department of Agriculture. Agricultural Research Service. Center for Grain and Animal Health Research. Manhatta, USA / University of Miami Miller School of Medicine. Department of Publica Health. Miami, FL, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.
University of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.
Abstract
The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, amajor challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection inmosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.
Keywords in Portuguese
Zika Virus
Aedes Aegypti
Espectroscopia de Luz Próxima ao Infravermelho
detecção não invasiva
 
Keywords
Zika Virus
Aedes aegypti
noninvasive detection
near-infrared spectroscopy
 
DeCS
Espectroscopia de Luz Próxima ao Infravermelho
Publisher
American Association for the Advancement of Science
Citation
FERNANDES, Jill N. et al. Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Sci. Adv., v.4, eaat0496, 6p, May 2018.
ISSN
2375-2548