Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27849
Title: Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia
Authors: Teles, Carolina Bioni Garcia
Santos, Ana Paula de Azevedo dos
Freitas, Rui Alves
Oliveira, Arley Faria José de
Ogawa, Guilherme Maerschner
Rodrigues, Moreno Souza
Pessoa, Felipe Arley Costa
Medeiros, Jansen Fernandes
Camargo, Luís Marcelo Aranha
Affilliation: Fundação Oswaldo Cruz. Porto Velho, RO, Brasil / Faculdade São Lucas. Porto Velho, RO, Brasil.
Universidade Federal de Rondônia. Porto Velho, RO, Brasil.
Instituto Nacional de Pesquisas da Amazônia. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Universidade de São Paulo. Instituto de Ciências Biomédicas. Monte Negro, RO, Brasil.
Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
Fundação Oswaldo Cruz. Porto Velho, RO, Brasil.
Faculdade São Lucas. Porto Velho, RO, Brasil / Universidade de São Paulo. Instituto de Ciências Biomédicas. Monte Negro, RO, Brasil.
Abstract: In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality.
Keywords: Sandflies
Leishmania
Disease vectors
Assis Brasil Municipality
Acre
Brazil
Issue Date: 2016
Publisher: Fundação Oswaldo Cruz
Citation: TELES, C.B.G. et al. Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, p. 423-432, 2016.
DOI: 10.1590/0074-02760160054
ISSN: 0074-0276
Copyright: open access
Appears in Collections:AM - ILMD - Artigos de Periódicos
RO - Artigos de Periódicos



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