Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/2798
Title: Montenegro’s skin reactions and antibodies against different Leishmania species in dogs from a visceral leishmaniosis endemic area
Authors: Baleeiro, Carolina de Oliveira
Silva, Moacir Paranhos
Santos, Juliana C. dos
Oliveira, Geraldo Gileno de Sá
Nascimento, Eliane Góes
Pontes-de-Carvalho, Lain Carlos
dosSantos, Washington Luis Conrado
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Laboratório Central do Estado da Bahia (LACEN). Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Centro de Referência em Endemias Pirajá da Silva (PIEJ), Jequie, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil
Abstract: In this study, humoral (circulating anti-Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti-L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D.=1.31) was significantly higher (ANOVA, P<0.01) than against L. amazonensis (mean O.D.=0.88) or L. braziliensis (mean O.D.=0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement (kappa=0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared (kappa=0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed (kappa=0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis.
Keywords: Leishmanin skin test
Leishmania chagasi
Leishmania braziliensis
Leishmania amazonensis
Leishmaniasis
Antibody-response
keywords: Leishmanin teste cutâneo
Leishmania chagasi
Leishmania braziliensis
Leishmania amazonensis
Leishmaniose
Anticorpo-resposta
DeCS: Análise de Variância
Animais
Anticorpos Antiprotozoários
Antígenos de Protozoários
Brasil
Doenças do Cão
Cães
ELISA
Leishmania
Leishmania braziliensis
Leishmaniose
Leishmaniose Cutânea
Leishmaniose Visceral
Filogenia
Estudos Soroepidemiológicos
Pele
Testes Cutâneos
Especificidade da Espécie
Issue Date: 2006
Publisher: Elsevier
Citation: BALEEIRO, C. O. et al. Montenegro’s skin reactions and antibodies against different Leishmania species in dogs from a visceral leishmaniosis endemic area. Veterinary Parasitology, v. 139, n. 1-3, p. 21-28, 2006.
Description: Agradecimentos ao Dr.Edson Duarte Moreira pela sua revisão cuidadosa da estatística e análise dos dados aqui apresentados.
DOI: 10.1016/j.vetpar.2006.02.033
ISSN: 0304-4017
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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