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Title: Evaluation of memory immune response to mycobacterium extract among household contact of tuberculosis cases
Authors: Cavalcanti, Y. V. N.
Pereira, V. R. A.
Reis, L. C.
Ramos, A. L. G.
Luna, C. F.
Nascimento, E. J. M.
Lucena-Silva, N.
Affilliation: Department of Immunology. Recife, PE, Brazil.
Department of Immunology. Recife, PE, Brazil.
Department of Immunology. Recife, PE, Brazil.
Department of Immunology. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Saúde Pública. Recife, PE, Brasil.
Laboratory of Virology and Experimental Therapy. Recife, PE, Brazil.
Department of Immunology. Recife, PE, Brazil.
Abstract: The human immune response to tuberculosis (TB) is especially mediated by T CD4(+)lymphocytes. However, more studies are needed in order to understand the exact role of each cytokine in the mechanisms for cures. In this article, our aim was to analyze the production of TNF-alpha, IL-10, and IFN-gamma in peripheral blood mononuclear cells (PBMCs) among the household contacts of common primary TB cases, with or without histories of active TB infection, who were negative to parasitological and HIV tests. In order to characterize the cytokine production, PBMCs from these groups were stimulated with whole-protein extract of M. tuberculosis (WPE) antigen (rAgTb) for 24 and 48 hr. The culture supernatants were collected and IFN-gamma, TNF-alpha, and IL-10 were assayed using capture ELISA. There were no statistical differences between primary TB cases and their household contacts with or without previous histories of lung TB. Our results suggest that T memory cells, T regulatory cells, and the Th1/Th2 dichotomy may be responsible for the results described in this article. Further studies are currently underway.
Keywords: tuberculosis
household contact
keywords: tuberculose
contato domiciliar
DeCS: Adolescente
Idoso, 80 anos ou mais
Antígenos Bacterianos / imunologia
Estudos de caso-controle
Células Cultivadas / imunologia
Citocinas / análise
Citocinas / imunologia
Exposição Ambiental
Memória Imunológica / imunologia
Interferon-gama / análise
Interferona-gama / imunologia
Interleucina-10 / análise
Interleucina-10 / imunologia
Leucócitos Mononucleares / imunologia
Meia idade
Mycobacterium tuberculosis / imunologia
Estatísticas não paramétricas
Teste de tuberculina
Tuberculose / imunologia
Tuberculose / microbiologia
Fator de Necrose Tumoral alfa / de análise
Fator de Necrose Tumoral alfa / imunologia
Issue Date: 2009
Citation: CAVALCANTI, Y. V. N. et al. Evaluation of Memory Immune Response to Mycobacterium Extract among Household Contact of Tuberculosis Cases. Journal of Clinical Laboratory Analysis, v. 23, n. 1, p. 57–62, 2009.
DOI: 10.1002/jcla.20290
ISSN: 1098-2825
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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