Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27997
Title: Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review
Authors: Paiva-Cavalcanti, M.
Regis-da-Silva, C.G.
Gomes, Y. M.
Affilliation: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Abstract: In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and may replace conventional PCR in diagnostic routines. Regarding visceral leishmaniasis, the possibility of deployment of real-time PCR in highly complex diagnoses (reference services) in endemic areas will facilitate a swift and safe return for patients. Moreover, the use of a technique that possesses elevated diagnostic sensitivity, and can monitor therapy and prevent relapses promotes broader prospects for the disease control.
Keywords: Diagnosis
Molecular diagnosis
Visceral leishmaniasis
keywords: Diagnóstico
Diagnóstico molecular
Leishmaniose visceral
DeCS: Reação em Cadeia da Polimerase
Leishmaniose Visceral / diagnóstico
Brasil / epidemiologia
Humanos
Sensibilidade e Especificidade
Issue Date: 2010
Citation: PAIVA-CAVALCANTI, M.; REGIS-DA-SILVA, C.; GOMES, Y. Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 16, n. 4, p. 537–542, 2010.
DOI: 10.1590/S1678-91992010000400004
ISSN: 1678-9199
Copyright: open access
Appears in Collections:PE - IAM - Artigos de Periódicos

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