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OSTEOBLASTS AND BONE MARROW MESENCHYMAL STROMAL CELLS CONTROL HEMATOPOIETIC STEM CELL MIGRATION AND PROLIFERATION IN 3D IN VITRO MODEL
Barros, Ana Paula D. N. de et al. | Date Issued: 2010
Author
Barros, Ana Paula D. N. de
Takiya, Christina M.
Garzoni, Luciana R.
Ferreira, Mona Lisa Leal
Dutra, Hélio S.
Chiarini, Luciana B.
Meirelles, Maria Nazareth
Borojevic, Radovan
Rossi, Maria Isabel D.
Affilliation
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Biofísica. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.
Abstract
Migration, proliferation, and differentiation of hematopoietic stem cells (HSCs) are dependent upon a complex three-dimensional (3D) bone marrow microenvironment. Although osteoblasts control the HSC pool, the subendosteal niche is complex and its cellular composition and the role of each cell population in HSC fate have not been established. In vivo models are complex and involve subtle species-specific differences, while bidimensional cultures do not reflect the 3D tissue organization. The aim of this study was to investigate in vitro the role of human bone marrow-derived mesenchymal stromal cells (BMSC) and active osteoblasts in control of migration, lodgment, and proliferation of HSCs.
Keywords in Portuguese
Osteoblastos
Medula óssea
Migração e proliferação
3 D
Células Estromais Mesenquimais
células-tronco hematopoiéticas
 
Keywords
Osteoblasts
Bone Marrow
hematopoietic stem cells
Mesenchymal Stromal Cells
Migration and Proliferation
3 D
 
Publisher
Public Library of Science
Citation
BARROS, Ana Paula D. N. de; et al. Osteoblasts and Bone Marrow Mesenchymal Stromal Cells Control Hematopoietic Stem Cell Migration and Proliferation in 3D In Vitro Model. PLoS ONE, v. 5, n.2, e9093, 13p, Feb. 2010.
DOI
10.1371/journal.pone.0009093
ISSN
1932-6203