Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/27999
Title: Histopathology and ultrastructure of midgut of Alabama argillacea (Hübner) (Lepidoptera: Noctuidae) fed Bt-cotton
Authors: Sousa, Maria Esmeralda C.
Santos, Fábio A. B.
Wanderley-Teixeira, Valéria
Teixeira, Alvaro A. C.
Siqueira, Herbert Álvaro A. de
Alves, Luiz C.
Torres, Jorge B.
Affilliation: Universidade Federal Rural de Pernambuco. Departamento de Agronomia. Programa de Pós-Graduação em Entomologia Agrícola. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade Federal Rural de Pernambuco. Departamento de Morfologia e Fisiologia Animal. Recife, PE, Brazil.
Universidade Federal Rural de Pernambuco. Departamento de Morfologia e Fisiologia Animal. Recife, PE, Brazil.
Universidade Federal Rural de Pernambuco. Departamento de Agronomia. Programa de Pós-Graduação em Entomologia Agrícola. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.
Universidade Federal Rural de Pernambuco. Departamento de Agronomia. Programa de Pós-Graduação em Entomologia Agrícola. Recife, PE, Brazil.
Abstract: The interaction of Cry toxins from Bacillus thuringiensis in the midgut of some insect larvae determines their efficacies as insecticides, due to the expression and availability of sites of action of the toxin in the midgut. Researches point out cases of resistance to Cry toxin due to alterations in the binding sites in columnar cell membrane. We analyzed the effects of Cry1Ac toxin expressed by Bt-cotton plants on Alabama argillacea midgut morphophysiology clarifying in levels of morphological and ultrastructural. Larvae in the 4th instar of A. argillacea after 20 min from ingesting Bt-cotton leaves expressing 0.183 ng of Cry1Ac exhibited ultrastructural and morphological modifications in the columnar cells with significant changes in the mitochondrial polymorphism, cytoplasmic vacuolization, microvillus and basal labyrinth. Expressive morphological alterations were also observed in the goblet cells indicating that the columnar cells are not the only target of the Cry1Ac toxin. The regenerative cells did not modify their structures and exhibited decrease in regeneration capacity. In conclusion, the ingestion of 0.183 ± 0.077 ng of Cry1Ac was enough to promote alterations in the columnar and goblet cells, besides reducing significantly the number of regenerative cells, which may have contributed to larval death. Nevertheless, further studies are necessary to determine the true cause of death.
Keywords: Bollgard
Lepidoptera
Microscopy
Cotton leafworm
Bacillus thuringiensis
keywords: Bollgard
Lepidoptera
Microscopia
Lençol de algodoeiro
Bacillus thuringiensis
DeCS: Animais
Proteínas Bacterianas / administração & dosagem
Proteínas Bacterianas / farmacologia
Distribuição Qui-Quadrado
Sistema Digestivo / metabolismo
Aparelho Digestivo / ultraestrutura
Endotoxinas / administração & dosagem
Endotoxinas / farmacologia
Gossípio / metabolismo
Proteínas Hemolisinas / administração & dosagem
Proteínas de hemolisina / farmacologia
Histocitoquímica
Larva / metabolismo
Larva / ultraestrutura
Lepidoptera / metabolismo
Lepidópteros / ultraestrutura
Microscopia Eletrônica
Controle de Pragas, Biológico / métodos
Plantas Geneticamente Modificadas / metabolismo
Plantas Geneticamente Modificadas / Ultraestrutura
Issue Date: 2010
Citation: SOUSA, Maria Esmeralda C. et al. Histopathology and Ultrastructure of Midgut of Alabama Argillacea (Hübner) (Lepidoptera: Noctuidae) Fed Bt-Cotton. Journal of Insect Physiology, v. 56, n. 12, p. 1913–1919, dez. 2010.
DOI: 10.1016/j.jinsphys.2010.08.014
ISSN: 1879-1611
Copyright: restricted access
Appears in Collections:PE - IAM - Artigos de Periódicos

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