Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/2802
Title: Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva
Authors: Menezes, Maria José
Costa, Dirceu Joaquim
Clarêncio, Jorge
Miranda, José Carlos
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz, Salvador, BA. Brasil
Abstract: Sand fly saliva contains potent and complex pharmacologic molecules that are able to modulate the host's hemostatic, inflammatory, and immune systems. In this study, we evaluated the effects of salivary gland sonicate (SGS) of Lutzomyia intermedia, the natural vector of Leishmania braziliensis, on monocytes obtained from the peripheral blood mononuclear cells (PBMC) of healthy volunteers. We investigated the effects of sand fly saliva on cytokine production and surface molecule expression of LPS-stimulated human monocytes uninfected or infected with L. braziliensis.Pre-treatment of non-infected human monocytes with L. intermedia SGS followed by LPS-stimulation led to a significant decrease in IL-10 production accompanied by a significant increase in CD86, CD80, and HLA-DR expression. Pre-treatment with SGS followed by LPS stimulation and L. braziliensis infection led to a significant increase in TNF-alpha, IL-6, and IL-8 production without significant alterations in co-stimulatory molecule expression. However, pre-treatment with L. intermedia SGS did not result in significant changes in the infection rate of human monocytes.Our data indicate that L. intermedia saliva is able to modulate monocyte response, and, although this modulation is dissociated from enhanced infection with L. braziliensis, it may be associated with successful parasitism
DeCS: Leishmaniose
Leishmania braziliensis
Monócitos
Psychodidae
Saliva
Citocinas
Citometria deFluxo
Issue Date: 2008
Citation: MENEZES, M. J. et al. Immunomodulation of human monocytes following exposure to Lutzomyia intermedia saliva. BMC Immunology, v. 9, n. 12, p. 1-8, 2008
DOI: 10.1186/1471-2172-9-12
ISSN: 1471-2172
Appears in Collections:IGM - Artigos de Periódicos

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