Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28177
Title: Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies
Authors: Michalsky, Erika Monteiro
Rocha, Marília Fonseca
Lima, Ana Cristina Vianna Mariano da Rocha
Silva, João Carlos França
Pires, Marize Quinhone
Oliveira, Fernanda Santos
Pacheco, Raquel Silva
Santos, Sara Lopes dos
Barata, Ricardo Andrade
Romanha, Alvaro José
Dias, Consuelo Latorre Fortes
Dias, Edelberto Santos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / Universidade Federal do Triângulo Mineiro. Uberaba, MG, Brasil.
Secretaria Municipal de Saúde. Montes Claros, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Abstract: Visceral leishmaniasis (VL) is a growing zoonosis with an increasing number of new cases and a rapid geographical spreading of the disease. In the present study, a canine survey was carried out in the city of Montes Claros (320,000 inhabitants), an endemic area of American visceral leishmaniasis in the state of Minas Gerais, Brazil. A total number of 4795 dogs were examined by serology, which showed a rate of seropositivity of 5%. Isoenzymatic analysis confirmed Leishmania infantum chagasi as the local aetiological agent of CVL. Canine tissues were assayed for the presence of Leishmania parasite DNA using different techniques. The infectivity of asymptomatic, oligosymptomatic and symptomatic seropositive dogs was tested by xenodiagnosis using laboratory reared Lutzomyia longipalpis. Rates of infection of 5.4%, 5.1% and 28.4% were found for the phlebotomine sand flies that fed in asymptomatic, oligosymptomatic and symptomatic dogs, respectively. Our results indicate that, under experimental conditions, symptomatic dogs are about four times more infective to VL vectors than oligosymptomatic or asymptomatic animals. The lower infectivity rates of dogs displaying any of the last two clinical forms of leishmaniasis, however, must be taken into account in the epidemiology of CVL.
Keywords: Canine visceral leishmaniasis
PCR
Leishmania chagasi
Lutzomyia longipalpis
Indirect immunofluorescence
Xenodiagnosis
Montes Claros, MG
keywords: Lutzomyia longipalpis
Reação em Cadeia da Polimerase
Leishmania infantum
Leishmaniose visceral canina
DeCS: Técnica Indireta de Fluorescência para Anticorpo
Xenodiagnóstico
Issue Date: 2007
Publisher: Elsevier 12 Months
Citation: MICHALSKY, Érika Monteiro; et al. Infectivity of seropositive dogs, showing different clinical forms of leishmaniasis, to Lutzomyia longipalpis phlebotomine sand flies. Veterinary Parasitology, v.147, p.67–76, 2007.
Description: Veterinary Parasitology Acesso aberto -Verde; Elsevier: 12 monthsVerde. Pode arquivar a versão preprint e postprint ou Versão/PDF do editor.
DOI: 10.1016/j.vetpar.2007.03.004
ISSN: 0304-4017
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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