Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/2819
Title: Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis
Authors: Moura, Tatiana Rodrigues de
Novais, Fernanda Oliveira
Oliveira, Fabiano
Clarêncio, Jorge
Almério, Noronha
Barral, Aldina Maria Prado
Brodskyn, Claudia Ida
Oliveira, Camila Indiani de
Affilliation: Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundacação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Abstract: Leishmania spp. cause a broad spectrum of diseases collectively known as leishmaniasis. Leishmania braziliensis is the main etiological agent of American cutaneous leishmaniasis (ACL) and mucocutaneous leishmaniasis. In the present study, we have developed an experimental model of infection that closely resembles ACL caused by L. braziliensis. In order to do so, BALB/c mice were infected in the ear dermis with 105 parasites and distinct aspects of the infection were evaluated. Following inoculation, parasite expansion in the ear dermis was accompanied by the development of an ulcerated dermal lesion which healed spontaneously, as seen by the presence of a scar. Histological analysis of infected ears showed the presence of a mixed inflammatory infiltrate consisting of both mononuclear and polymorphonuclear cells. In draining lymph nodes, parasite replication was detected throughout the infection. In vitro restimulation of draining lymph node cells followed by intracellular staining showed an up-regulation in the production of gamma interferon (IFN- ) and in the frequency of IFN- -secreting CD4 and CD8 T cells. Reverse transcription-PCR of ears and draining lymph node cells showed the expression of CC chemokines. The dermal model of infection with L. braziliensis herein is able to reproduce aspects of the natural infection, such as the presence of an ulcerated lesion, parasite dissemination to lymphoid areas, and the development of a Th1-type immune response. These results indicate that this model shall be useful to address questions related to the concomitant immunity to reinfection and parasite persistence leading to mucocutaneous leishmaniasis
keywords: Animais
Linfócitos T CD4-Positivos
Linfócitos T CD8-Positivos
Quimiocinas CC
Derme/PS/PA
Modelos Animais de Doenças
Orelha Externa/IM/PS/PA
Feminino
Interferon gama/ME
Leishmania braziliensis
Leishmaniose Cutânea
Linfonodos/ME/PS/PA
Camundongos
Camundongos Endogâmicos BALB C
Issue Date: 2005
Citation: MOURA, T. R. de et al. Toward a novel experimental model of infection to study American cutaneous leishmania braziliensis. Infectium Immunity, v.73, n.9, p.5827-34, 2005.
DOI: 1098-5522
ISSN: doi:10.1128/IAI.73.9.5827–5834.2005
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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