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TRYPANOSOMA EVANSI: MOLECULAR HOMOGENEITY AS INFERRED BY PHENETICAL ANALYSIS OF RIBOSOMAL INTERNAL TRANSCRIBED SPACERS DNA OF AN ECLECTIC PARASITE
Reação em Cadeia da Polimerase
Homogeneidade molecular
Análise fenética de DNA
Parasita eclético
Espaçadores transcritos internos ribossômicos
PCR
Molecular homogeneity
Phenetical analysis
Eclectic parasite
Ribosomal DNA
Internal transcribed spacer
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Abstract
The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5.8S ribosomal subunit further submitted to restriction enzymes digestion were carried out in DNAs extracted from 41 T. evansi strains isolated from horses, dogs, coatis and capybaras from two distinct regions of the Brazilian Pantanal. We also used one T. evansi isolate from Africa, one from Asia and one isolate of T. b. brucei from Africa. Analysis of the RFLP profiles yielded a unique "riboprinting" that does not vary intraspecifically. These results provide insights on the ribosomal gene organization of T. evansi and showed that ITS analysis by RFLP show high genetic similarity of this locus among isolates of this protozoan parasite.
Keywords in Portuguese
Trypanosoma evansiReação em Cadeia da Polimerase
Homogeneidade molecular
Análise fenética de DNA
Parasita eclético
Espaçadores transcritos internos ribossômicos
Keywords
Trypanosoma evansiPCR
Molecular homogeneity
Phenetical analysis
Eclectic parasite
Ribosomal DNA
Internal transcribed spacer
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