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BONE MARROW MONONUCLEAR CELLS ATTENUATE INTERSTITIAL FIBROSIS AND STIMULATE THE REPAIR OF TUBULAR EPITHELIAL CELLS AFTER UNILATERAL URETERAL OBSTRUCTION
Células-Tronco Adultas
Obstrução ureteral unilateral
Reparação de tecidos
Células mononucleares da medula óssea
Unilateral ureteral obstruction
Tissue repair
Nephropathies
Adult stem cells
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Abstract
The growing number of patients suffering from chronic
renal disease is a challenge for the development of
innovative therapies. Benefits of cell therapy in acute
renal diseases in animal models have been reported
but seldom for chronic lesions. We present evidence
for the improvement of renal morphology in a model
of tubulointerstitial fibrosis. Wistar rats were submitted
to unilateral ureteral obstruction (UUO), treated with
bone-marrow mononuclear cells (UUO+BMMC)
infused via the cava vein, and killed on day 14.
Labeled BMMC were seen in renal tissue after 7 days
in the group UUO+BMMC. UUO+BMMC also showed
a reduction in ED1+ cells and tubular apoptotic cells
together with enhanced tubular proliferation.
Myofibroblasts were also reduced after BMMC which
is consistent with a decrease in collagen deposition
(picro Sirius staining) and RT-PCR data showing lower
levels of procollagen-I mRNA. Simultaneously, nestin+
cells increased in the interstitium and decreased in
the tubules. Double stained nestin+/α-SMA+ cells were
present only in the interstitium, and their levels did
not change after BMMC infusion. These data indicate
a renoprotective effect of BMMC through increased
tubular cell regeneration, inhibition of tubular cell
apoptosis and partially blocking of the inflammatory
and fibrotic events that occur after unilateral ureteral
obstruction.
Keywords in Portuguese
NefropatiasCélulas-Tronco Adultas
Obstrução ureteral unilateral
Reparação de tecidos
Células mononucleares da medula óssea
Keywords
Bone marrow mononuclear cellsUnilateral ureteral obstruction
Tissue repair
Nephropathies
Adult stem cells
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