Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28391
Title: Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virus serotypes 1 or 2
Authors: Barreto, Débora Ferreira
Schatzmayr, Hermann Gonçalves
Takiya, Christina Maeda
Jácome, Fernanda Cunha
Silva, Manoel Enderson Vieira
Faria, Nieli Rodrigues da Costa
Nogueira, Rita Maria Ribeiro
Barth, Ortrud Monika
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Departamento de Histologia e Embriologia,. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ. Brasil.
Abstract: Histological and ultrastructural alterations in lung of BALB/c mice reinfected with heterologous DENV by the intravenous route with non-neuroadapted dengue viruses serotypes 1 and 2 were analyzed. The lung samples were processed following the standard techniques of photonic and transmission electron microscopy. Morphological studies showed breakdown of alveolocapilar barrier leading to alveolitis, focal zones of collapse, and intraalveolar hemorrhage. Inside alveolar septa, congested capillaries exhibited neutrophils and platelets. Alveolar capillary endothelial cells exhibited aspects of activation with exuberant phylopodia and intracytoplasmic vesicles and vacuoles. Morphometrical analyses demonstrated an increase of the surface density of interalveolar septa in all reinfected mice while alveolar space density was decreased. All these morphometrical data corroborated the histological features emphasizing the alveolocapilar breakdrown in dengue infected animals. Important is the fact that reinfection leads to intraalveolar fibrogenesis as demonstrated by histomorphometry and ultrastructural studies. DENV particles, antigens and RNA were observed 72 hours post-reinfection in mosquito cells (C6/36) inoculated with sera of the animals. The morphological alterations observed in lungs were similar to the observed in human cases of dengue fever and dengue hemorrhagic fever. The present study demonstrates that the BALB/c mice, during the secondary infection by a heterologous serotype of DENV, develop morphological alterations in lung tissue more severe than those observed in the primary infection.
Keywords: dengue-1 virus
dengue-2 virus
BALB/c mice
lung
histopathology
ultrastructure
keywords: Vírus da Dengue tipo 1
Vírus da Dengue tipo 2
Pulmão
histopatologia
Ultraestrutura
Camundongos BALB / c
Issue Date: 2009
Publisher: Sociedade Brasileira de Virologia
Citation: BARRETO, Débora Ferreira; et al. Histopathological and ultrastructural studies of lung tissue of mice reinfected with dengue virys serotypes 1 or 2. Virus Reviews & Research, v.14, n.2, 20p, 2009.
ISSN: 1519-2563
Copyright: open access
Appears in Collections:IOC - Artigos de Periódicos

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