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https://www.arca.fiocruz.br/handle/icict/28684
LOWER GALACTOSYLATION LEVELS OF THE LIPOPHOSPHOGLYCAN FROM LEISHMANIA (LEISHMANIA) MAJOR-LIKE STRAINS AFFECT INTERACTION WITH PHLEBOTOMUS PAPATASI AND LUTZOMYIA LONGIPALPIS
lipofosfoglicano
Interação parasita-hospedeiro
Phlebotomus papatasi
Lutzomyia longipalpis
lipophosphoglycan
host-parasite interaction
Phlebotomus papatasi
Lutzomyia longipalpis
Author
Affilliation
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Charles University. Faculty of Science. Department of Parasitology. Prague, Czech Republic
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil
Abstract
BACKGROUND: Leishmania major is an Old World species causing cutaneous leishmaniasis and is transmitted by Phlebotomus papatasi and Phlebotomus duboscqi. In Brazil, two isolates from patients who never left the country were characterised as L. major-like (BH49 and BH121). Using molecular techniques, these isolates were indistinguishable from the L. major reference strain (FV1).
OBJECTIVES; We evaluated the lipophosphoglycans (LPGs) of the strains and their behaviour in Old and New World sand fly vectors.
METHODS: LPGs were purified, and repeat units were qualitatively evaluated by immunoblotting. Experimental in vivo infection with L. major-like strains was performed in Lutzomyia longipalpis (New World, permissive vector) and Ph. papatasi(Old World, restrictive or specific vector).
FINDINGS: The LPGs of both strains were devoid of arabinosylated side chains, whereas the LPG of strain BH49 was more galactosylated than that of strain BH121. All strains with different levels of galactosylation in their LPGs were able to infect both vectors, exhibiting colonisation of the stomodeal valve and metacyclogenesis. The BH121 strain (less galactosylated) exhibited lower infection intensity compared to BH49 and FV1 in both vectors.
MAIN CONCLUSIONS: Intraspecific variation in the LPG of L. major-like strains occur, and the different galactosylation levels affected interactions with the invertebrate host.
Keywords in Portuguese
Leishmania majorlipofosfoglicano
Interação parasita-hospedeiro
Phlebotomus papatasi
Lutzomyia longipalpis
Keywords
Leishmania majorlipophosphoglycan
host-parasite interaction
Phlebotomus papatasi
Lutzomyia longipalpis
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