Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28696
Title: Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi
Authors: Cavalcanti, Danielle Pereira
Shimada, Márcia Kiyoe
Probst, Christian Macagnan
Souto-Padrón, Thais Cristina Baeta Soares
Souza, Wanderley de
Goldenberg, Samuel
Fragoso, Stenio Perdigão
Motta, Maria Cristina Machado
Affilliation: Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Universidade Católica Dom Bosco. Campo Grande, MS, Brasil.
Universidade Federal do Paraná. Curitiba, PR, Brasil.
Universidade Federal do Mato Grosso do Sul. Campo Grande, MS, Brasil.
Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.
Embrapa Gado de Corte. Saúde Animal. Laboratório de Engenharia Genética Animal, Campo Grande, MS, Brasil.
Abstract: he kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network is similar to that of the entire cell. However, within the kinetoplast matrix, the kDNA is highly condensed. Studies in Crithidia fasciculata showed that kinetoplast-associated proteins (KAPs) are capable of condensing the kDNA network. However, little is known about the KAPs of Trypanosoma cruzi, a parasitic protozoon that shows distinct patterns of kDNA condensation during their complex morphogenetic development. In epimastigotes and amastigotes (replicating forms) the kDNA fibers are tightly packed into a disk-shaped kinetoplast, whereas trypomastigotes (non-replicating) present a more relaxed kDNA organization contained within a rounded structure. It is still unclear how the compact kinetoplast disk of epimastigotes is converted into a globular structure in the infective trypomastigotes. In this work, we have analyzed KAP coding genes in trypanosomatid genomes and cloned and expressed two kinetoplast-associated proteins in T. cruzi: TcKAP4 and TcKAP6. Such small basic proteins are expressed in all developmental stages of the parasite, although present a differential distribution within the kinetoplasts of epimastigote, amastigote and trypomastigote forms. This way concluded thas several features of TcKAPs, such as their small size, basic nature and similarity with KAPs of C. fasciculata, are consistent with a role in DNA charge neutralization and condensation. Additionally, the differential distribution of KAPs in the kinetoplasts of distinct developmental stages of the parasite, indicate that the kDNA rearrangement that takes place during the T. cruzi differentiation process is accompanied by TcKAPs redistribution.
Keywords: DNA
Kinetoplast
Cloning
Gene Expression
Keywords in spanish: ADN de Cinetoplasto
Clonación Molecular
Expresión Génica
keywords: Trypanosoma cruzi
Crithidia fasciculata
DeCS: DNA de Cinetoplasto
Clonagem Molecular
Expressão Gênica
Issue Date: 2009
Publisher: Springer Nature
Citation: CAVALCANTI, Danielle Pereira et al. Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi. BMC Microbiology, v. 9, n. 120, 2009.
DOI: 10.1186/1471-2180-9-120
ISSN: 1471-2180
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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