Author | Alessio, Glaucia Diniz | |
Author | Araujo, Fernanda Fortes de | |
Author | Sales Junior, Policarpo Ademar | |
Author | Gomes, Matheus de Souza | |
Author | Amaral, Laurence Rodrigues do | |
Author | Xavier, Marcelo Antonio Pascoal | |
Author | Carvalho, Andrea Teixeira de | |
Author | Lana, Marta de | |
Author | Martins Filho, Olindo Assis | |
Access date | 2018-09-17T12:19:39Z | |
Available date | 2018-09-17T12:19:39Z | |
Document date | 2018 | |
Citation | ALESSIO, Glaucia Diniz et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018. | pt_BR |
ISSN | 1935-2735 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28813 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | doenças parasitárias | pt_BR |
Subject in Portuguese | Árvores de decisão | pt_BR |
Subject in Portuguese | doença de chagas | pt_BR |
Subject in Portuguese | sorodiagnostico | pt_BR |
Subject in Portuguese | sangue | pt_BR |
Subject in Portuguese | Citometria de fluxo | pt_BR |
Subject in Portuguese | tripomastigotas | pt_BR |
Title | Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0006140 | |
Abstract | The methods currently available for genotype-specific diagnosis of T. cruzi infection still present relevant limitations, especially to identify mixed infection. In the present investigation, we have evaluated the performance of Chagas-Flow ATE-IgG2a test for early and late differential diagnosis of single and dual genotype-specific T. cruzi infections. Serum samples from Swiss mice at early and late stages of T. cruzi infection were assayed in parallel batches for genotype-specific diagnosis of single (TcI, TcVI or TcII) and dual (TcI+TcVI, TcVI+TcII or TcII+TcI) infections. The intrinsic reactivity to TcI, TcVI and TcII target antigens, including amastigote (AI/AVI/AII), trypomastigote-(TI/TVI/TII) and epimastigote (EI/ EVI/EII), at specific reverse of serum dilutions (500 to 64,000), was employed to provide reliable decision-trees for ªearlyº vs ªlateº, ªsingle vs ªdualº and ªgenotype-specificº serology. The results demonstrated that selective set of attributes ªEII 500/EI 2,000/AII 500º were able to provide high-quality accuracy (81%) to segregate early and late stages of T. cruzi infection. The sets ªTI 2,000/AI 1,000/EII 1,000º and ªTI 8,000/AII 32,000º presented expressive scores to discriminate single from dual T. cruzi infections at early (85%) and late stages (84%), respectively. Moreover, the attributes ªTI 4,000/TVI 500/TII 1,000º, ªTI 16,000/EI 2,000/EII 2,000/AI 500/TVI 500º showed good performance for genotype-specific diagnosis at early stage of single (72%) and dual (80%) T. cruzi infections, respectively. In addition, the attributes ªTI 4,000/AII 1,000/EVI 1,000º, ªTI 64,000/AVI 500/AI 2,000/AII 1,000/EII 4,000º showed moderate performance for genotype-specific diagnosis at late stage of single (69%) and dual (76%) T. cruzi infections, respectively. The sets of decision-trees were assembled to construct a sequential algorithm with expressive accuracy (81%) for serological diagnosis of T. cruzi infection. These findings engender new perspectives for the application of Chagas-Flow ATE-IgG2a method for genotype-specific diagnosis in humans, with relevant contributions for epidemiological surveys as well as clinical and post-therapeutic monitoring of Chagas disease. | pt_BR |
Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pos-graduacão em Sanidade e Producão Animal nos Tropicos. Uberaba, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doencas Infecciosas e Parasitarias. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | Parasitic diseases | pt_BR |
Subject | Decision trees | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | Serodiagnosis | pt_BR |
Subject | Blood | pt_BR |
Subject | Flow cytometry | pt_BR |
Subject | Trypomastigotes | pt_BR |