Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28813
Title: Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
Authors: Alessio, Glaucia Diniz
Araujo, Fernanda Fortes de
Sales Junior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antonio Pascoal
Carvalho, Andrea Teixeira de
Lana, Marta de
Martins Filho, Olindo Assis
Affilliation: Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pos-graduacão em Sanidade e Producão Animal nos Tropicos. Uberaba, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
Universidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doencas Infecciosas e Parasitarias. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
Abstract: The methods currently available for genotype-specific diagnosis of T. cruzi infection still present relevant limitations, especially to identify mixed infection. In the present investigation, we have evaluated the performance of Chagas-Flow ATE-IgG2a test for early and late differential diagnosis of single and dual genotype-specific T. cruzi infections. Serum samples from Swiss mice at early and late stages of T. cruzi infection were assayed in parallel batches for genotype-specific diagnosis of single (TcI, TcVI or TcII) and dual (TcI+TcVI, TcVI+TcII or TcII+TcI) infections. The intrinsic reactivity to TcI, TcVI and TcII target antigens, including amastigote (AI/AVI/AII), trypomastigote-(TI/TVI/TII) and epimastigote (EI/ EVI/EII), at specific reverse of serum dilutions (500 to 64,000), was employed to provide reliable decision-trees for ªearlyº vs ªlateº, ªsingle vs ªdualº and ªgenotype-specificº serology. The results demonstrated that selective set of attributes ªEII 500/EI 2,000/AII 500º were able to provide high-quality accuracy (81%) to segregate early and late stages of T. cruzi infection. The sets ªTI 2,000/AI 1,000/EII 1,000º and ªTI 8,000/AII 32,000º presented expressive scores to discriminate single from dual T. cruzi infections at early (85%) and late stages (84%), respectively. Moreover, the attributes ªTI 4,000/TVI 500/TII 1,000º, ªTI 16,000/EI 2,000/EII 2,000/AI 500/TVI 500º showed good performance for genotype-specific diagnosis at early stage of single (72%) and dual (80%) T. cruzi infections, respectively. In addition, the attributes ªTI 4,000/AII 1,000/EVI 1,000º, ªTI 64,000/AVI 500/AI 2,000/AII 1,000/EII 4,000º showed moderate performance for genotype-specific diagnosis at late stage of single (69%) and dual (76%) T. cruzi infections, respectively. The sets of decision-trees were assembled to construct a sequential algorithm with expressive accuracy (81%) for serological diagnosis of T. cruzi infection. These findings engender new perspectives for the application of Chagas-Flow ATE-IgG2a method for genotype-specific diagnosis in humans, with relevant contributions for epidemiological surveys as well as clinical and post-therapeutic monitoring of Chagas disease.
Keywords: Trypanosoma cruzi
Parasitic diseases
Decision trees
Chagas disease
Serodiagnosis
Blood
Flow cytometry
Trypomastigotes
keywords: Trypanosoma cruzi
doenças parasitárias
Árvores de decisão
doença de chagas
sorodiagnostico
sangue
Citometria de fluxo
tripomastigotas
Issue Date: 2018
Publisher: Public Library of Science
Citation: ALESSIO, Glaucia Diniz et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018.
DOI: 10.1371/journal.pntd.0006140
ISSN: 1935-2735
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

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