Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28824
Title: Usefulness of FC-TRIPLEX Chagas/Leish IgG1 as confirmatory assay for non-negative results in blood bank screening of Chagas disease
Authors: Campos, Fernanda Magalhães Freire
Repoles, Laura Cotta
Araújo, Fernanda Fortes de
Pascoal, Vanessa Peruhype Magalhães
Xavier, Marcelo Antônio Pascoal
Sabino, Ester Cerdeira
Proietti, Anna Bárbara de Freitas Carneiro
Andrade, Mariléia Chaves
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Gontijo, Célia Maria Ferreira
Affilliation: Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pós graduação em Sanidade e Produção Animal nos Trópicos, Uberaba, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Pró-Sangue. São Paulo, SP, Brazil/Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brazil
Fundação Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Montes Claros, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz Instituto René Rachou. Belo Horizonte, MG, Brazil
Abstract: A relevant issue in Chagas disease serological diagnosis regards the requirement of using several confirmatory methods to elucidate the status of non-negative results from blood bank screening. The development of a single reliable method may potentially contribute to distinguish true and false positive results. Our aim was to evaluate the performance of the multiplexed flow-cytometry anti-T. cruzi/Leishmania IgG1 serology/(FC-TRIPLEX Chagas/Leish IgG1) with three conventional confirmatory criteria (ELISA-EIA, Immunofluorescence assay-IIF and EIA/IIF consensus criterion) to define the final status of samples with actual/previous non-negative results during anti-T. cruzi ELISA-screening in blood banks. Apart from inconclusive results, the FC-TRIPLEX presented a weak agreement index with EIA, while a strong agreement was observed when either IIF or EIA/IIF consensus criteria were applied. Discriminant analysis and Spearman's correlation further corroborates the agreement scores. ROC curve analysis showed that FC-TRIPLEX performance indexes were higher when IIF and EIA/IIF consensus were used as a confirmatory criterion. Logistic regression analysis further demonstrated that the probability of FC-TRIPLEX to yield positive results was higher for inconclusive results from IIF and EIA/IIF consensus. Machine learning tools illustrated the high level of categorical agreement between FC-TRIPLEX versus IIF or EIA/IIF consensus. Together, these findings demonstrated the usefulness of FC-TRIPLEX as a tool to elucidate the status of non-negative results in blood bank screening of Chagas disease.
Keywords: Blood bank
Chagas disease
Confirmatory assay
FC-TRIPLEX Chagas/Leish IgG1
Flow cytometry
Serology
keywords: banco de sangue
Doença de chagas
Ensaio confirmatório
FC-TRIPLEX Chagas/Leish IgG1
citometria de fluxo
sorologia
Issue Date: 2018
Publisher: Elsevier Inc
Citation: CAMPOS, Fernanda Magalhães Freire et al. Usefulness of FC-TRIPLEX Chagas/Leish IgG1 as confirmatory assay for non-negative results in blood bank screening of Chagas disease. J Immunol Methods., v. 455, p. 34-40, 2018
DOI: 10.1016/j.jim.2018.01.006
ISSN: 0022-1759
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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