| Author | Silveira, Guilherme Ferreira | |
| Author | Wowk, Pryscilla F. | |
| Author | Cataneo, Allan H. D. | |
| Author | Santos, Paula F. dos | |
| Author | Delgobo, Murilo | |
| Author | Stimamiglio, Marco A. | |
| Author | Sarzi, Maria Lo | |
| Author | Thomazelli, Ana Paula F. S. | |
| Author | Conchon-Costa, Ivete | |
| Author | Pavanelli, Wander Rogerio | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Báfica, André | |
| Author | Mansur, Daniel S. | |
| Author | Santos, Claudia N. Duarte dos | |
| Author | Bordignon, Juliano | |
| Access date | 2018-09-18T12:30:27Z | |
| Available date | 2018-09-18T12:30:27Z | |
| Document date | 2018 | |
| Citation | SILVEIRA, Guilherme Ferreira et al. Human T Lymphocytes Are Permissive for Dengue Virus Replication. J Virol., v. 92, n. 10, pii: e02181-17, 2018 | pt_BR |
| ISSN | 0022-538X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28847 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | linfocitos T | pt_BR |
| Subject in Portuguese | Dengue virus | pt_BR |
| Subject in Portuguese | replicação | pt_BR |
| Title | Human T Lymphocytes Are Permissive for Dengue Virus Replication. | pt_BR |
| Type | Article | |
| DOI | 10.1128/JVI.02181-17 | |
| Abstract | Dengue virus (DV) infection can cause either a self-limiting flu-like disease or a threatening hemorrhage that may evolve to shock and death. A variety of cell types, such as dendritic cells, monocytes, and B cells, can be infected by DV. However, despite the role of T lymphocytes in the control of DV replication, there remains a paucity of information on possible DV-T cell interactions during the disease course. In the present study, we have demonstrated that primary human naive CD4+ and CD8+ T cells are permissive for DV infection. Importantly, both T cell subtypes support viral replication and secrete viable virus particles. DV infection triggers the activation of both CD4+ and CD8+ T lymphocytes, but preactivation of T cells reduces the susceptibility of T cells to DV infection. Interestingly, the cytotoxicity-inducing protein granzyme A is highly secreted by human CD4+ but not CD8+ T cells after exposure to DV in vitro Additionally, using annexin V and polycaspase assays, we have demonstrated that T lymphocytes, in contrast to monocytes, are resistant to DV-induced apoptosis. Strikingly, both CD4+ and CD8+ T cells were found to be infected with DV in acutely infected dengue patients. Together, these results show that T cells are permissive for DV infection in vitro and in vivo, suggesting that this cell population may be a viral reservoir during the acute phase of the disease.IMPORTANCE Infection by dengue virus (DV) causes a flu-like disease that can evolve to severe hemorrhaging and death. T lymphocytes are important cells that regulate antibody secretion by B cells and trigger the death of infected cells. However, little is known about the direct interaction between DV and T lymphocytes. Here, we show that T lymphocytes from healthy donors are susceptible to infection by DV, leading to cell activation. Additionally, T cells seem to be resistant to DV-induced apoptosis, suggesting a potential role as a viral reservoir in humans. Finally, we show that both CD4+ and CD8+ T lymphocytes from acutely infected DV patients are infected by DV. Our results raise new questions about DV pathogenesis and vaccine development. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Imunobiologia. Florianópolis, S C, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Imunobiologia. Florianópolis, S C, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil | pt_BR |
| Affilliation | Prefeitura de Cambé. Secretaria Municipal de Saúde. Saúde da Família. Cambé, PR, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Protozoologia Experimental. Londrina, PR, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Protozoologia Experimental. Londrina, PR, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Protozoologia Experimental. Londrina, PR, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Imunobiologia. Florianópolis, SC, Brazil | pt_BR |
| Affilliation | Universidade Federal de Santa Catarina. Laboratório de Imunobiologia. Florianópolis, SC, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brazil | pt_BR |
| Subject | T lymphocytes | pt_BR |
| Subject | dengue virus | pt_BR |
| Subject | replication | pt_BR |
| Embargo date | 2024-01-01 | |
