Author | Silveira, Guilherme Ferreira | |
Author | Meyer, Florencia | |
Author | Delfraro, Adriana | |
Author | Mosimann, Ana Luiza Pamplona | |
Author | Coluchi, Norma | |
Author | Vasquez, Cyntia | |
Author | Probst, Christian Macagnan | |
Author | Báfica, André | |
Author | Bordignon, Juliano | |
Author | Santos, Claudia Nunes Duarte dos | |
Access date | 2018-09-19T16:45:53Z | |
Available date | 2018-09-19T16:45:53Z | |
Document date | 2011 | |
Citation | SILVEIRA, Guilherme F. et al. Dengue Virus Type 3 Isolated from a Fatal Case with Visceral Complications Induces Enhanced Proinflammatory Responses and Apoptosis of Human Dendritic Cells. Journal of Virology, v. 85, n. 11, p. 5374–5383, 2011. | pt_BR |
ISSN | 0022-538X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/28897 | |
Description | O artigo encontra-se disponível em acesso aberto no site do Editor. | pt_BR |
Language | por | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Dengue | pt_BR |
Title | Dengue virus type 3 isolated from a fatal case with visceral complications induces enhanced proinflammatory responses and apoptosis of human dendritic cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/JVI.01915-10 | |
Abstract | A recent (2007 to 2009) dengue outbreak caused by dengue virus (DENV) in Paraguay presented unusual severe clinical outcomes associated with 50% mortality rates. Although it has been reported that inflammatory responses influence the severity of dengue virus infection (T. Pang, M. J. Cardosa, and M. G. Guzman, Immunol. Cell Biol. 85:43-45, 2007), there remains a paucity of information on virus-innate immunity interactions influencing clinical outcome. Using human dendritic cells from a major innate immune cell population as an in vitro model, we have investigated signature cytokine responses as well as infectivity-replicative profiles of DENV clinical isolates from either a nonfatal case of classical dengue fever (strain DENV3/290; isolated in Brazil in 2002) or a fatal case of dengue fever with visceral complications isolated in Paraguay in 2007 (strain DENV3/5532). Strain DENV3/5532 was found to display significantly higher replicative ability than DENV3/290 in monocyte-derived dendritic cells (mdDCs). In addition, compared to DENV3/290 results, mdDCs exposed to DENV3/5532 showed increased production of proinflammatory cytokines associated with higher rates of programmed cell death, as shown by annexin V staining. The observed phenotype was due to viral replication, and tumor necrosis factor alpha (TNF-α) appears to exert a protective effect on virus-induced mdDC apoptosis. These results suggest that the DENV3/5532 strain isolated from the fatal case replicates within human dendritic cells, modulating cell survival and synthesis of inflammatory mediators. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil / Universidade de la Republica. Facultad de Ciencias. Sección Virologia, Montevideo, Uruguai. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay. | pt_BR |
Affilliation | Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Laboratório de Imunofarmacologia e Doenças Infecciosas. Florianópolis, SC, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Subject | Dendritic Cells | pt_BR |
Subject | Cytokines | pt_BR |
Subject | Dengue Virus | pt_BR |
Subject | Pyroptosis | pt_BR |
Subject in Spanish | Células Dendríticas | pt_BR |
Subject in Spanish | Citocinas | pt_BR |
Subject in Spanish | Virus del Dengue | pt_BR |
Subject in Spanish | Piroptosis | pt_BR |
DeCS | Células Dendríticas | pt_BR |
DeCS | Citocinas | pt_BR |
DeCS | Vírus da Dengue | pt_BR |
DeCS | Piroptose | pt_BR |
e-ISSN | 1098-5514 | |