Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28897
Title: Dengue virus type 3 isolated from a fatal case with visceral complications induces enhanced proinflammatory responses and apoptosis of human dendritic cells
Authors: Silveira, Guilherme Ferreira
Meyer, Florencia
Delfraro, Adriana
Mosimann, Ana Luiza Pamplona
Coluchi, Norma
Vasquez, Cyntia
Probst, Christian Macagnan
Báfica, André
Bordignon, Juliano
Santos, Claudia Nunes Duarte dos
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil / Universidade de la Republica. Facultad de Ciencias. Sección Virologia, Montevideo, Uruguai.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay.
Instituto de Previsión Social. Laboratorio Central de Salud Publica. Servicio de Pediatria, Asuncion, Paraguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.
Universidade Federal de Santa Catarina. Laboratório de Imunofarmacologia e Doenças Infecciosas. Florianópolis, SC, Brazil
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Abstract: A recent (2007 to 2009) dengue outbreak caused by dengue virus (DENV) in Paraguay presented unusual severe clinical outcomes associated with 50% mortality rates. Although it has been reported that inflammatory responses influence the severity of dengue virus infection (T. Pang, M. J. Cardosa, and M. G. Guzman, Immunol. Cell Biol. 85:43-45, 2007), there remains a paucity of information on virus-innate immunity interactions influencing clinical outcome. Using human dendritic cells from a major innate immune cell population as an in vitro model, we have investigated signature cytokine responses as well as infectivity-replicative profiles of DENV clinical isolates from either a nonfatal case of classical dengue fever (strain DENV3/290; isolated in Brazil in 2002) or a fatal case of dengue fever with visceral complications isolated in Paraguay in 2007 (strain DENV3/5532). Strain DENV3/5532 was found to display significantly higher replicative ability than DENV3/290 in monocyte-derived dendritic cells (mdDCs). In addition, compared to DENV3/290 results, mdDCs exposed to DENV3/5532 showed increased production of proinflammatory cytokines associated with higher rates of programmed cell death, as shown by annexin V staining. The observed phenotype was due to viral replication, and tumor necrosis factor alpha (TNF-α) appears to exert a protective effect on virus-induced mdDC apoptosis. These results suggest that the DENV3/5532 strain isolated from the fatal case replicates within human dendritic cells, modulating cell survival and synthesis of inflammatory mediators.
Keywords: Dendritic Cells
Cytokines
Dengue Virus
Pyroptosis
Keywords in spanish: Células Dendríticas
Citocinas
Virus del Dengue
Piroptosis
keywords: Dengue
DeCS: Células Dendríticas
Citocinas
Vírus da Dengue
Piroptose
Issue Date: 2011
Publisher: American Society for Microbiology
Citation: SILVEIRA, Guilherme F. et al. Dengue Virus Type 3 Isolated from a Fatal Case with Visceral Complications Induces Enhanced Proinflammatory Responses and Apoptosis of Human Dendritic Cells. Journal of Virology, v. 85, n. 11, p. 5374–5383, 2011.
DOI: 10.1128/JVI.01915-10
ISSN: 0022-538X
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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