| Author | Costa, Jimena Ferreira da | |
| Author | Ferrarini, Mariana Galvão | |
| Author | Nardelli, Sheila Cristina | |
| Author | Goldenberg, Samuel | |
| Author | Ávila, Andréa Rodrigues | |
| Author | Holetz, Fabíola Barbieri | |
| Access date | 2018-09-20T12:10:31Z | |
| Available date | 2018-09-20T12:10:31Z | |
| Document date | 2018 | |
| Citation | COSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28924 | |
| Language | por | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Title | Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760170531 | |
| Abstract | Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. As methods, we used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. It finded that TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. This way we have as main conclusions that, TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | RNA | pt_BR |
| Subject | Messenger | pt_BR |
| Subject | Ribonucleoproteins | pt_BR |
| Subject | Gene Expression | pt_BR |
| Subject in Spanish | ARN Mensajero | pt_BR |
| Subject in Spanish | Expresión Génica | pt_BR |
| DeCS | RNA Mensageiro | pt_BR |
| DeCS | Ribonucleoproteínas | pt_BR |
| DeCS | Expressão Gênica | pt_BR |
| e-ISSN | 1678-8060 | |
