Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/28924
Title: Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
Authors: Costa, Jimena Ferreira da
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
Affilliation: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Abstract: Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. As methods, we used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. It finded that TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. This way we have as main conclusions that, TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.
Keywords: RNA
Messenger
Ribonucleoproteins
Gene Expression
Keywords in spanish: ARN Mensajero
Expresión Génica
keywords: Trypanosoma cruzi
DeCS: RNA Mensageiro
Ribonucleoproteínas
Expressão Gênica
Issue Date: 2018
Publisher: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
Citation: COSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018.
DOI: 10.1590/0074-02760170531
ISSN: 0074-0276
Copyright: open access
Appears in Collections:PR - ICC - Artigos de Periódicos

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