| Author | Lima, Cristiana Soares de | |
| Author | Marques, Maria A. M. | |
| Author | Debrie, Anne-Sophie | |
| Author | Almeida, Elza C. C. | |
| Author | Silva, Carlos A. M. | |
| Author | Brennan, Patrick J. | |
| Author | Sarno, Euzenir Nunes | |
| Author | Menozzi, Franco D. | |
| Author | Pessolani, Maria Cristina V. | |
| Access date | 2018-09-20T15:10:56Z | |
| Available date | 2018-09-20T15:10:56Z | |
| Document date | 2009 | |
| Citation | LIMA, Cristiana Soares de; et al. Heparin-binding hemagglutinin(HBHA)ofMycobacteriumleprae is expressed during infectionand enhances bacterial adherence to epithelial cells. FEMS Microbiol Lett., v.292, p.162–169, 2009. | pt_BR |
| ISSN | 0378-1097 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/28940 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Células Epiteliais | pt_BR |
| Subject in Portuguese | Adesinas | pt_BR |
| Subject in Portuguese | hemaglutinina de ligação a heparina | pt_BR |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | imunogenicidade. | pt_BR |
| Title | Heparin-binding hemagglutinin (HBHA) of Mycobacterium leprae is expressed during infection and enhances bacterial adherence to epithelial cells | pt_BR |
| Type | Article | |
| DOI | 10.1111/j.1574-6968.2009.01488.x | |
| Abstract | A heparin-binding hemagglutinin (HBHA) expressed on the surface of Mycobacterium tuberculosis is an antigenic protein that has been implicated in bacterial adherence to epithelial cells and systemic dissemination. In this study, the potential role of the Mycobacterium leprae HBHA (ML-HBHA) homologue in leprosy was investigated. Initially, the in vivo expression of HBHA and its association with the M. leprae cell envelope was confirmed by immunoblotting and proteomic analysis. Mycobacterium leprae recombinant HBHA (rML-HBHA) bound to a heparin-Sepharose column, and its capacity to act as an adhesin was demonstrated in experiments showing that the exogenous addition of the protein to latex beads or to M. leprae cells promotes a dramatic increase in association with epithelial cells. Finally, serum anti-HBHA immunoglobulin G levels were investigated in individuals infected with M. leprae. Altogether, our data indicate that HBHA is recognized during the course of bacterial infection in humans and may play a role in leprosy pathogenesis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Affilliation | INSERM U629. Lille, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Institut Pasteur de Lille. Lille, France / IFR142. Lille, France. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | heparin-binding hemagglutinin | pt_BR |
| Subject | adhesin | pt_BR |
| Subject | epithelial cell | pt_BR |
| Subject | leprosy | pt_BR |
| Subject | immunogenecity. | pt_BR |
| e-ISSN | 1574-6968 | |
| Embargo date | 2030-01-01 | |
