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2030-01-01
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- IOC - Artigos de Periódicos [12339]
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HEPARIN-BINDING HEMAGGLUTININ (HBHA) OF MYCOBACTERIUM LEPRAE IS EXPRESSED DURING INFECTION AND ENHANCES BACTERIAL ADHERENCE TO EPITHELIAL CELLS
Células Epiteliais
Adesinas
hemaglutinina de ligação a heparina
Hanseníase
imunogenicidade.
heparin-binding hemagglutinin
adhesin
epithelial cell
leprosy
immunogenecity.
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
INSERM U629. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Institut Pasteur de Lille. Lille, France / IFR142. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
INSERM U629. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA / Institut Pasteur de Lille. Lille, France / IFR142. Lille, France.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
Abstract
A heparin-binding hemagglutinin (HBHA) expressed on the surface of Mycobacterium tuberculosis is an antigenic protein that has been implicated in bacterial adherence to epithelial cells and systemic dissemination. In this study, the potential role of the Mycobacterium leprae HBHA (ML-HBHA) homologue in leprosy was investigated. Initially, the in vivo expression of HBHA and its association with the M. leprae cell envelope was confirmed by immunoblotting and proteomic analysis. Mycobacterium leprae recombinant HBHA (rML-HBHA) bound to a heparin-Sepharose column, and its capacity to act as an adhesin was demonstrated in experiments showing that the exogenous addition of the protein to latex beads or to M. leprae cells promotes a dramatic increase in association with epithelial cells. Finally, serum anti-HBHA immunoglobulin G levels were investigated in individuals infected with M. leprae. Altogether, our data indicate that HBHA is recognized during the course of bacterial infection in humans and may play a role in leprosy pathogenesis.
Keywords in Portuguese
Mycobacterium lepraeCélulas Epiteliais
Adesinas
hemaglutinina de ligação a heparina
Hanseníase
imunogenicidade.
Keywords
Mycobacterium lepraeheparin-binding hemagglutinin
adhesin
epithelial cell
leprosy
immunogenecity.
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