Author | Lafouresse, Fanny | |
Author | Almeida, Vinicius Cotta de | |
Author | Malet-Engra, Gema | |
Author | Galy, Anne | |
Author | Valitutti, Salvatore | |
Author | Dupré, Loïc | |
Access date | 2018-11-08T12:37:38Z | |
Available date | 2018-11-08T12:37:38Z | |
Document date | 2012 | |
Citation | LAFOURESSE, Fanny; et al. Wiskott–Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1. Immunology, v.137, p.183–196, 2012. | pt_BR |
ISSN | 0019-2805 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/29937 | |
Language | eng | pt_BR |
Publisher | Wiley 12 Months | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | varredura de células apresentadoras de antígenos | pt_BR |
Subject in Portuguese | antígeno associado à função linfocitária 1 | pt_BR |
Subject in Portuguese | Motilidade das células T | pt_BR |
Subject in Portuguese | proteína da síndrome de wiskott-Aldrich | pt_BR |
Title | Wiskott–Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1 | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/j.1365-2567.2012.03620.x | |
Abstract | T-cell scanning for antigen-presenting cells (APC) is a finely tuned process.
Whereas non-cognate APC trigger T-cell motility via chemokines
and intercellular adhesion molecule-1 (ICAM-1), cognate APC deliver a
stop signal resulting from antigen recognition. We tested in vitro the contribution
of the actin cytoskeleton regulator Wiskott–Aldrich syndrome
protein (WASP) to the scanning activity of primary human CD4+ T cells.
WASP knock-down resulted in increased T-cell motility upon encounter
with non-cognate dendritic cells or B cells and reduced capacity to stop
following antigen recognition. The high motility of WASP-deficient T cells
was accompanied by a diminished ability to round up and to stabilize
pauses. WASP-deficient T cells migrated in a normal proportion towards
CXCL12, CCL19 and CCL21, but displayed an increased adhesion and
elongation on ICAM-1. The elongated morphology of WASP-deficient
T cells was related to a reduced confinement of high-affinity lymphocyte
function-associated antigen 1 to the mid-cell zone. Our data therefore
indicate that WASP controls CD4+ T-cell motility upon APC
encounter by regulating lymphocyte function-associated antigen 1 spatial
distribution. | pt_BR |
Affilliation | INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France. | pt_BR |
Affilliation | INSERM, U951. Généthon, Evry, France. | pt_BR |
Affilliation | INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France. | pt_BR |
Affilliation | INSERM, U1043 / Université Toulouse III Paul-Sabatier. Centre de Physiopathologie de Toulouse Purpan. 3CNRS, U5282, Toulouse, France. | pt_BR |
Subject | antigen-presenting cell scanning | pt_BR |
Subject | lymphocyte function-associated antigen 1 | pt_BR |
Subject | T-cell motility | pt_BR |
Subject | Wiskott–Aldrich syndrome protein | pt_BR |
e-ISSN | 1365-2567 | |
Embargo date | 2030-01-01 | |