Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/29966
Title: Characterization of coagulase-negative staphylococci isolated from hospital indoor air and a comparative analysis between airborne and inpatient isolates of Staphylococcus epidermidis
Authors: Botelho, Ana Maria Nunes
Nunes, Zilma das Grac¸a
Asensi, Marise Dutra
Gomes, Marisa Zenaide Ribeiro
Fracalanzza, Sérgio Eduardo Longo
Figueiredo, Agnes Marie Sá
Affilliation: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goés. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Universidade do Estado do Rio de Janeiro. Hospital Pedro Ernesto. Laboratório de Bacteriologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ. Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goés. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goés. Departamento de Microbiologia Médica. Rio de Janeiro, RJ, Brasil.
Abstract: A total of 108 coagulase-negative staphylococci (CoNS) were collected from hospital indoor air. The majority of the isolates were able to produce biofilms and displayed multiresistance profiles. The most frequent species identified were Staphylococcus epidermidis (n527) and Staphylococcus haemolyticus (n517). Potential virulence traits (icaAD, aap, hld, atlE and sesB) and genotypic profiles were compared for S. epidermidis isolates from indoor air (n527) and from patients (n526) who had been admitted to the hospital 8–34 months after air sampling. Overall, the virulence factors tested were more frequently found among S. epidermidis recovered from clinical origin than from air sources (P50.003). Indeed, the group of patient isolates exhibited superior ability to accumulate biofilms (P,0.0001). Despite this, genotyping using PFGE revealed that identical clones of S. epidermidis could be recovered from both patient and indoor air samples. In addition, some airborne isolates displayed virulence profiles and levels of biofilm accumulation similar to those found in patient isolates. Therefore, further studies are necessary to clarify the importance of hospital indoor air as a route of transmission for CoNS isolates (mainly S. epidermidis).
Keywords: Staphylococcus epidermidis
coagulase-negative staphylococci
hospital indoor
comparative analysis
inpatients
keywords: Staphylococcus epidermidis
Análise comparativa
Ambiente hospitalar
estafilococos coagulase-negativos
Internação
Pacientes Internados
Issue Date: 2012
Publisher: Microbiology Society
Citation: BOTELHO, Ana Maira Nunes; et al. Characterization of coagulase-negative staphylococci isolated from hospital indoor air and a comparative analysis between airborne and inpatient isolates of Staphylococcus epidermidis. Journal of Medical Microbiology, v. 61, p.1136-1145, 2012.
DOI: 10.1099/jmm.0.035931-0
ISSN: 0022-2615
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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