Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30123
Title: Trophoblast cells are able to regulate monocyte activity to control Toxoplasma gondii infection
Authors: Castro, Andressa da Silva
Alves, Celene Maria de Oliveira Simões
Angeloni, Mariana Bodini
Gomes, Angélica Oliveira
Barbosa, Bellisa de Freitas
Franco, Priscila Silva
Silva, Deise Aparecida de Oliveira
Martins Filho, Olindo Assis
Mineo, José Roberto
Mineo, Tiago Wilson Patriarca
Ferro, Eloisa Amália Vieira
Affilliation: Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil/Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratoro de Imunoparasitologia. Uberlândia, MG, Brazil
Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Histologia e Embriologia. Uberlândia, MG, Brazil
Abstract: Introduction: Toxoplasma gondii is an intracellular parasite that causes severe disease when the infection occurs during pregnancy. Trophoblast cells constitute an important maternal–fetal barrier, with monocytes concentrating around them. Thus, interactions between trophoblasts and monocytes are important for maintaining a successful pregnancy, especially in cases of infection. This study aimed to evaluate the role of trophoblast cells (BeWo line) on monocyte (THP-1 line) activity in the presence or absence of T. gondii infection. Methods: THP-1 cells were stimulated with supernatants of BeWo cells, previously infected or not with T. gondii, and then infected with parasites. The supernatant of both cells were collected and analyzed for cytokine production and T. gondii proliferation in THP-1 cells was determined. Results: The results showed that after infection, the pattern of cytokines secreted by THP-1 and BeWo cells was characterized as a pro-inflammatory profile. Furthermore, supernatant of BeWo cells infected or not, was able to change the cytokine profile secreted by infected THP-1 cells, and this supernatant became THP-1 cells more able to control T. gondii proliferation than those that had not been stimulated. Discussion: This effect was associated with secretion of interleukin (IL)-6 by the THP-1 cells and soluble factors secreted by BeWo cells, such as IL-6 and MIF. Conclusion: Together, these results suggest that trophoblast cells are able to modulate monocyte activity, resulting in the control of T. gondii infection and subsequent maintenance of pregnancy.
Keywords: Trophoblast cell
Monocytes
Toxoplasma gondii
Cytokines
keywords: Célula Trofoblástica
Monócitos
Toxoplasma gondii
citocinas
Issue Date: 2013
Publisher: Elsevier
Citation: CASTRO, Andressa da Silva et al. Trophoblast cells are able to regulate monocyte activity to control Toxoplasma gondii infection. Placenta, v. 34, n. 3, p. 240-7, 2013
DOI: 10.1016/j.placenta.2012.12.006
ISSN: 0143-4004
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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