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HIGH THROUGHPUT SCREENING OF HYDROLYTIC ENZYMES FROM TERMITES USING A NATURAL SUBSTRATE DERIVED FROM SUGARCANE BAGASSE
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Background: The description of new hydrolytic enzymes is an important step in the development of techniques
which use lignocellulosic materials as a starting point for fuel production. Sugarcane bagasse, which is subjected to
pre-treatment, hydrolysis and fermentation for the production of ethanol in several test refineries, is the most
promising source of raw material for the production of second generation renewable fuels in Brazil. One problem
when screening hydrolytic activities is that the activity against commercial substrates, such as
carboxymethylcellulose, does not always correspond to the activity against the natural lignocellulosic material.
Besides that, the macroscopic characteristics of the raw material, such as insolubility and heterogeneity, hinder its
use for high throughput screenings.
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