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2030-01-01
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PC PROMOTER FROM CLASS 2 INTEGRONS AND THE CASSETTE TRANSCRIPTION PATTERN IT EVOKES
Fonseca, Érica Lourenço da et al. | Date Issued: 2011
Author
Fonseca, Érica Lourenço da
Freitas, Fernanda dos Santos
Vicente, Ana Carolina Paulo
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ. Brasil.
Abstract
Integrons are considered expression systems due to the presence of Pc promoters that drive gene cassette transcription. The role and configurations of Pc are well known in class 1 integrons; however, this region has not yet been identified in class 2 integrons. This study aimed to characterize the Pc promoter from class 2 integrons and to determine the effect of gene cassette position on transcription driven by this promoter.
Keywords in Portuguese
Klebsiella pneumoniae
Vibrio cholerae
Reação em Cadeia da Polimerase em Tempo Real
Integrons
 
Keywords
intI2
real-time RT–PCR
5′ RACE
Klebsiella pneumoniae
Vibrio cholerae
 
Publisher
Oxford University Press
Citation
FONSECA, Érica Lourenço da; FREITAS, Fernanda dos Santos; VICENTE, Ana Carolina Paulo. Pc promoter from class 2 integrons and the cassette transcription pattern it evokes. J. Antimicrob Chemother., v.66, p.797-801, 2011.
DOI
10.1093/jac/dkr011
ISSN
0305-7453